We narrowed to 10,561 results for: fus
-
Plasmid#175295PurposeTrack mature FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with SNAP-tag
TagsSNAP-tagExpressionMammalianAvailable SinceOct. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cx26-msfGFP
Plasmid#69016PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB451
Plasmid#74833PurposeGateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM-Venus
Plasmid#164785PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Gaussia princeps)
TagsGLuc and mVenusExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-mNG
Plasmid#187843PurposeConstitutive mNeonGreen-expression vector for Fusobacterium nucleatumDepositorInsertmNeonGreen
ExpressionBacterialAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-spCas9-SB
Plasmid#169919PurposeExpression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.DepositorInsertSpCas9-SB100X
UseCRISPRPromoterCAGAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D.R
Plasmid#51510PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSPromoter2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW-IRF4-Puro
Plasmid#123323PurposeLentiviral vector for expression of IRF4 under a Doxycycline inducible promoterDepositorInsertIRF4 (IRF4 Human)
UseLentiviralExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterDoxycycline inducible minimal CMVAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
LDN
Plasmid#21195DepositorUseRetroviralMutationMek1 K97M fused to ER*, catalytically inactiveAvailable SinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
hyA3A-BE4max
Plasmid#157943PurposeMammalian expression of hyA3A-BE4maxDepositorInserthyA3A-BE4max
UseCRISPRExpressionMammalianAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
TagsAll FPs are fused to H2BExpressionMammalianPromoterCAGAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Mfn1-E209A
Plasmid#141155PurposeMammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysisDepositorInsertMFN 1 (MFN1 Human)
TagsAcGFPExpressionMammalianMutationchanged Glutamic acid at position 209 to Alanine …PromoterCMVAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUPI hT5-S322-Cyp
Plasmid#80282PurposeExpresses human TRIM5 CypA fusionDepositorAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant mNG-ORP8
Plasmid#195170Purposemammalian expression of siRNA-resistant ORP8 tagged with mNeonGreenDepositorInsertORP8 (OSBPL8 Human)
TagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from K174 t…PromoterCMVAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL70008
Plasmid#50336DepositorInsertpFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107319PurposeExpresses SpCas9 fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to nuclease dead SaCas9 (D10A, N5…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB34
Plasmid#122245PurposeLentivirus delivery for stable expression of LbCas12a-VPRDepositorInsertmammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator
UseLentiviralTagsNLS and VPR activatorPromoterEFsAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK1036
Plasmid#17728DepositorAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
BB3cH_pGAP_23*_pPFK300_Cas9
Plasmid#104912PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-UFD1L
Plasmid#31560DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only