Purpose
QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.
Insert
QUAS-T7-TetO-GFP ssrA-T7 Stop- T7-LacO-TetR-T7 Stop
Tags
ssrA degradation tag (DAS+4)
Available Since
Oct. 14, 2022
Availability
Academic Institutions and Nonprofits only