We narrowed to 7,720 results for: ALP

1. pUCM-AAVS1-TO-hNGN2

   Plasmid

   #105840

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mCherry
   rtTA3G
   

   Use

   CRISPR and TALEN

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_HNF4A_P2A_Hygro_Barcode

   Plasmid

   #120450

   Purpose

   Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. BACE2_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176942

   Purpose

   Mammalian expression vector encoding BACE2 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   BACE2 (BACE2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-N-Flag-NLRP3

   Plasmid

   #75127

   Purpose

   Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiPGK Puro DEST p38KTRClover

   Plasmid

   #59152

   Purpose

   Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E-Runx1+23

   Plasmid

   #69602

   Purpose

   Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

   Depositor

   Owen Tamplin, Leonard Zon

   Article

   Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

   Inserts

   Runx1 +23 intronic enhancer (Runx1 Mouse)
   Mouse beta-globin minimal promoter
   

   Use

   5' gateway entry vector

   Promoter

   C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cre-IRES-PuroR

   Plasmid

   #30205

   Purpose

   Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

   Depositor

   Darrell Kotton

   Article

   Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

   Insert

   Cre recombinase (cre Enterobacteria phage P1)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   April 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-RUNX2

   Plasmid

   #192926

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   RUNX2 (RUNX2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2546

   Plasmid

   #144209

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CEBPA (CEBPA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. anxA2-GFP

    Plasmid

    #107196

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A2 (ANXA2 Human)
    

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Ala residue 65 in human sequence replaced by Glu …

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0658

    Plasmid

    #143722

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CHD3 (CHD3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. BII-CMV-AfmW3A

    Plasmid

    #133402

    Purpose

    Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Afamin and Wnt3a (Afm, Wnt3a Mouse)
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hPDGFRA-GFP

    Plasmid

    #22919

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Sac-Abeta(M1-42)

    Plasmid

    #71875

    Purpose

    Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-42) (APP Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

    Plasmid

    #120565

    Purpose

    Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, T35A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

    Plasmid

    #120567

    Purpose

    Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, Y40A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

    Plasmid

    #120566

    Purpose

    Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, E37A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MLRV

    Plasmid

    #118069

    Purpose

    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Inserts

    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Expression

    Mammalian

    Promoter

    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag_HsIRE1a_pBabePuro

    Plasmid

    #54337

    Purpose

    N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    The endogenous IRE1a signal sequence (aa1-18) was…

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Sac-Abeta(M1-40)

    Plasmid

    #71876

    Purpose

    Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-40) (APP Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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