We narrowed to 7,293 results for: Ank

1. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

   Plasmid

   #229816

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBOBI N-FLAG HIPK2

   Plasmid

   #213004

   Purpose

   expresses mouse HIPK2

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   HIPK2 (Hipk2 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-lox-inv[Talpha1-HA-Dre]-lox-Akna-mOrange2

   Plasmid

   #196893

   Purpose

   Neuron-specific expression of Akna fused to mOrange2. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Akna-mOrange2

   Plasmid

   #196880

   Purpose

   Neuron-specific expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pINSPECT:NEAT1-total_SigP:NLuc

   Plasmid

   #197267

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   NEAT1-total homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Expression

   Mammalian

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9

   Plasmid

   #196897

   Purpose

   Neuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-Akap9(128-425) (Akap9 Rat)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

   Plasmid

   #196894

   Purpose

   Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   N-gTBD-mOrange2 (NEDD1 Human)
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

   Plasmid

   #196895

   Purpose

   Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-CAMSAP3 (Camsap3 Mouse)
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBactin-mOrange2-Cdk5rap2-CTD

   Plasmid

   #196855

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid

    #196856

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    

    Expression

    Mammalian

    Mutation

    Lacking the centrosome-targeting domain (CTD)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pD649-HAsp-LRRN2-COMP5AP-AviTag-9xHis

    Plasmid

    #157492

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    LRRN2 (LRRN2 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV6-MPP8-delta600-728-10xHis

    Plasmid

    #194183

    Purpose

    Expresses MPP8-delta600-728_10xHis

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-delta600-728 (4xANK deletion)

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEXSISERS_NLuc_FRT-PuroR

    Plasmid

    #177865

    Purpose

    Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    NLuc-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Tags

    OLLAS

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEXSISERS_BSD_FRT-PuroR

    Plasmid

    #177867

    Purpose

    Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    BSD-based EXSISERS
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    OLLAS

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEXSISERS_SurfaceHaloTag_FRT-PuroR

    Plasmid

    #177869

    Purpose

    Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    Surface-HaloTag-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#2

    Plasmid

    #173986

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #2 mutated
    

    Use

    Luciferase

    Mutation

    Coordinator motif mutation #2

    Promoter

    SV40 promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-noSV40-EC1.45-human_min1-opossum_min2

    Plasmid

    #173966

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Mutation

    See Depositor Comments Below

    Promoter

    SV40 promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

    Plasmid

    #173994

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated
    

    Use

    Luciferase

    Mutation

    7X Coordinator motif mutation #1-2-3-4-5-6-7

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

    Plasmid

    #173962

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs
    

    Use

    Luciferase

    Mutation

    4 mutated Coordinator motifs

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#1

    Plasmid

    #173985

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #1 mutated
    

    Use

    Luciferase

    Mutation

    Coordinator motif mutation #1

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge