We narrowed to 10,125 results for: Uty

1. pJT119b

   Plasmid

   #50551

   Purpose

   Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

   Inserts

   CcaS
   CcaR
   sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR-Hyg

   Plasmid

   #158708

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Expression

   Bacterial

   Promoter

   Hsp60

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPL5606_TEF1*-Cre_ADE2

   Plasmid

   #60932

   Purpose

   Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Promoter

   mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. psd44-G12QL

   Plasmid

   #46825

   Purpose

   expression of mutant G protein alpha 12 in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   GNA12 (GNA12 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Q231L (the mutation reduces GTPase activity resul…

   Promoter

   PGK

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSHDY-PvanCC-mVenus_vanR

   Plasmid

   #137664

   Purpose

   Conjugative vector derived from pSHDY. Constitutive expression of the repressor vanR, as well as vanillate-inducible expression of mVenus.

   Depositor

   Ilka Axmann

   Article

   Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

   Insert

   PvanCC:mVenus
   

   Expression

   Bacterial

   Promoter

   PvanCC

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

   Plasmid

   #92400

   Purpose

   Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

   Depositor

   Periklis Pantazis

   Article

   Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

   Inserts

   Dendra2
   Cerulean
   

   Use

   Zebrafish expression

   Tags

   H2B tag and membrane tag

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPL5608_TEF1*-Cre_TRP1

   Plasmid

   #60931

   Purpose

   Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Promoter

   Mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTarget12f1

   Plasmid

   #171183

   Purpose

   Constitutive expression of sgRNA without donor DNA

   Depositor

   Kohsuke Honda

   Article

   Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

   Insert

   sgRNA scaffold in the CRISPR/Cas12f1 system
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI) promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEXP5-NT/pT7 LasI

    Plasmid

    #193631

    Purpose

    Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LasI
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 RNAP promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKL09-GCaMP6f-mScarlet bb118

    Plasmid

    #158979

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-mScarlet fusion
    

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB1A3-AD011

    Plasmid

    #116852

    Purpose

    Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

    Depositor

    Friedrich Simmel

    Article

    Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

    Inserts

    AraC
    mScarletI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LT1_33_pTEFmut8-mCherry

    Plasmid

    #177706

    Purpose

    Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEFmut8-mCherry
    

    Use

    Used as donor dna in genome integration after lin…

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEXP5-NT/pT7 LuxI

    Plasmid

    #193626

    Purpose

    Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LuxI
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 RNAP promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM117

    Plasmid

    #127212

    Purpose

    BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbS9a-RFP

    Plasmid

    #126172

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23113

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mscv2.2-NAIP1

    Plasmid

    #60200

    Purpose

    Mouse NAIP1 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NAIP1

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    NAIP1
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CAG-LSL-mCherry-shPtbp1

    Plasmid

    #178591

    Purpose

    Cre-dependent expression of mCherry and a shRNA against Ptbp1 under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Ptbp1
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mscv2.2-NAIP2

    Plasmid

    #60201

    Purpose

    Mouse NAIP2 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NAIP2

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    NAIP2 (Naip2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD100 VP16-ZF43

    Plasmid

    #138761

    Purpose

    Constitutive expression of VP16-ZF43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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