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pcDNA 3.2/V5-DEST NET1A V5 WT

Plasmid

#69817

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

NET1A (NET1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-MCS-cytoBirA-2A-mCherry_Ras

Plasmid

#80058

Purpose

MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Tags

3x HA

Expression

Bacterial

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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B4GALT1-Dox

Plasmid

#124633

Purpose

Doxycycline-inducible B4GALT1 expression in mammalian cells

Depositor

Ron Weiss

Article

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Beta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635

Expression

Mammalian

Promoter

TRE-tight and hEF1a

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

Plasmid

#107727

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-Pav-GFP

Plasmid

#24286

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Pavarotti (pav Fly)

Tags

GFP

Expression

Insect

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

Plasmid

#214877

Purpose

Lentiviral vector encoding RfxCas13d targeting LPT guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crLPT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX nsp1 K164A/H165A CoV2

Plasmid

#175514

Purpose

For protein expression of K164A/H165A nsp1 CoV2

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

K164A/H165A nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX nsp1 R124A/K125A CoV2

Plasmid

#175513

Purpose

For protein expression of R124A/K125A nsp1 CoV2

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

R124A/K125A nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX C_K nsp1 K164A/H165A CoV2

Plasmid

#175517

Purpose

Expresses C_K for bioconjugation on nsp1

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

C_K nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AdMKP-3 S197A

Plasmid

#131522

Purpose

Expresses MKP-3 with S197A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Mutation

changed Serine 197 to Alanine

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSico-shRheb

Plasmid

#81087

Purpose

conditional expression of an shRNA targeting murine Rheb

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Rheb shRNA (Rheb Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSIN-CD4

Plasmid

#175942

Purpose

knockout mouse CD4

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

CD4 gRNA (Cd4 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-flk1-cytoBirA-2A-mCherry_Ras

Plasmid

#80056

Purpose

Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Tags

3x HA

Expression

Bacterial

Promoter

flk1

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-RORb2

Plasmid

#73999

Purpose

Overexpression of RORb2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

RORb2 ORF (Rorb Mouse)

Tags

none

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xML1N(7Q)

Plasmid

#67666

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1Nmut*2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Gfi1b

Plasmid

#131592

Purpose

doxycycline-inducible expression of mouse GFI1B in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Gfi1b (Gfi1b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-Blunt-U6-HES7-STOP-gRNA

Plasmid

#130933

Purpose

gRNA for tag human HES7

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7-STOP-gRNA (HES7 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-RORb2-IRES-GFPd2

Plasmid

#73998

Purpose

Plasmid expressing RORb2-IRES-GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

RORb2 ORF (Rorb Mouse)

Tags

none

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO human shRNA 1 c-MAF

Plasmid

#29432

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

c-MAF shRNA 1 (MAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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