We narrowed to 15,697 results for: NOL

1. YE2-BE4max

   Plasmid

   #138156

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YE2)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRetroX-Tight-MCS_PGK-OgNLuc

   Plasmid

   #70186

   Purpose

   Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNICKclos1.0

   Plasmid

   #73639

   Purpose

   Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sGRNA to pyrE
   

   Use

   E.coli-clostridium shuttle vector

   Mutation

   D10A

   Promoter

   j23119 and ptb

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCH68 (dAsCas12a-3xKRAB piggyBac)

   Plasmid

   #217334

   Purpose

   piggyBac expression of dAsCas12a-3xKRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   dAsCas12a
   

   Use

   CRISPR

   Tags

   nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E993A

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pClodANL-FGF4

   Plasmid

   #107874

   Purpose

   Bacterial expression of FGF4-NanoLuc

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Insert

   FGF4 (FGF4 Synthetic)
   

   Use

   Luciferase

   Tags

   NanoLuc

   Expression

   Bacterial

   Promoter

   pro D

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCpf1-BE

   Plasmid

   #107685

   Purpose

   Expresses dLbCpf1-BE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE
   

   Expression

   Mammalian

   Mutation

   D832A/E925A/D1148A

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA NIA Null

   Plasmid

   #198728

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs
   

   Expression

   Plant

   Promoter

   pU6-26, pU6-29

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pv6_CBX1_2xChromo

   Plasmid

   #179393

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY2ShHELIX_sgRNA_entry (CJT30)

   Plasmid

   #181781

   Purpose

   Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Expression

   Bacterial

   Mutation

   Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB510

    Plasmid

    #74852

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGI3EM17

    Plasmid

    #210569

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Promoter

    SpH2Bpro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB415

    Plasmid

    #74809

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB453

    Plasmid

    #74835

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-ZoAl-RTD-3xF-ORF

    Plasmid

    #238486

    Purpose

    For production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    DD644/645->AA

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-ZoAl-END-3xF-ORF

    Plasmid

    #238485

    Purpose

    For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    DD1041/1054->AA

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-TaGu-RTD-3xF-ORF

    Plasmid

    #238490

    Purpose

    For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    DD660/661->AA

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-TaGu-END-3xF-ORF

    Plasmid

    #238489

    Purpose

    For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    DD1057/1070->AA

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTLR repair vector

    Plasmid

    #64322

    Purpose

    Vector for repair of the traffic light reporter (TLR), providing the venus coding region

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    Venus
    

    Use

    Artificial targeting vector

    Mutation

    see publication for details

    Promoter

    none

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO-EGFP-RIP2

    Plasmid

    #131201

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

    Insert

    RIPK2 (RIPK2 Human)
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-HLA-A2_GFP

    Plasmid

    #85161

    Purpose

    HLA.A2_GFP fusion with 2 glycines in between

    Depositor

    Tone Fredsvik Gregers, Sébastien Walchli

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Gateway entry vector

    Tags

    EGFP

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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