We narrowed to 28,597 results for: Cas9

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pLenti-eGFP-dSaCas9-blast

Plasmid

#191653

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

Depositor

Moshe Szyf

Publication

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

eGFP-T2A-dSaCas9

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pvPE-V3

Plasmid

#240287

Purpose

Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

Depositor

Lusheng Huang

Publication

Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pvPE-V3

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pvPE-V3

Plasmid

#240287

Purpose

Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

Depositor

Lusheng Huang

Publication

Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pvPE-V3

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBK1290-AAV-dSaCas9

Plasmid

#223139

Purpose

AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Publication

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1290-AAV-dSaCas9

Plasmid

#223139

Purpose

AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Publication

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-SMCR8

Plasmid

#83440

Purpose

Expresses Cas9 and a gRNA targeting SMCR8

Depositor

Shawn Ferguson

Publication

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human SMCR8 (SMCR8 Human)

Expression

Mammalian

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-SMCR8

Plasmid

#83440

Purpose

Expresses Cas9 and a gRNA targeting SMCR8

Depositor

Shawn Ferguson

Publication

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human SMCR8 (SMCR8 Human)

Expression

Mammalian

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

Plasmid

#174139

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

Depositor

Benjamin Kleinstiver

Insert

human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-mScarlet

Expression

Mammalian

Mutation

VRQR=D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

Plasmid

#174139

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

Depositor

Benjamin Kleinstiver

Insert

human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-mScarlet

Expression

Mammalian

Mutation

VRQR=D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS Donor mClover-LMNA

Plasmid

#98970

Purpose

Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

Depositor

Jan Karlseder

Publication

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)

Expression

Mammalian

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCAGGS Donor mClover-LMNA

Plasmid

#98970

Purpose

Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

Depositor

Jan Karlseder

Publication

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)

Expression

Mammalian

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Publication

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sc++

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Publication

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sc++

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75240

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75241

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75240

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75241

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

Plasmid

#107731

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Publication

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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