We narrowed to 7,375 results for: ef1a

1. pAAV-EF1a-FDIO-ArchT-GFP

   Plasmid

   #124640

   Purpose

   AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   ArchT
   

   Use

   AAV; Flp/frt

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

   Plasmid

   #159756

   Purpose

   PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EF1a-SPdCas9-EGFP-2A-Blast

   Plasmid

   #71215

   Purpose

   dCas9 fused to EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-EGFP
   

   Use

   Lentiviral

   Tags

   dSpCas9-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-FLEX-ArchT-GFP

   Plasmid

   #58851

   Purpose

   AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-GFP
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-Ef1a-RPS27A-UTR-PP7-WPRE

   Plasmid

   #198339

   Purpose

   PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   RPS27A-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1a-mTagBFP-T2A-TetOn3G

   Plasmid

   #179888

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   mTagBFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lv EF1a HP1cs-Frb1x PGK Puro

   Plasmid

   #102808

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   HP1cs-Frb1x
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA

   Plasmid

   #37087

   Purpose

   Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   NpHR3.0-EYFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

   Plasmid

   #170240

   Purpose

   Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S218D, S222D

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

    Plasmid

    #203845

    Purpose

    Flp-dependent enzymatically inactive Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Enzymatically inactive Cre

    Promoter

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pb459-mU6-sgRNA-EF1a-PuroR

    Plasmid

    #195507

    Purpose

    piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Puromycin
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. P-Ef1a-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170324

    Purpose

    Expresses V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

    Plasmid

    #166411

    Purpose

    express hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    ef-1a

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

    Plasmid

    #71217

    Purpose

    dCas9 fused to human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B catalytic domain
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-MinE-IRES-Blast

    Plasmid

    #213115

    Purpose

    Expression of untagged MinE in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE (minE )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC446 - pAAV EF1a hPREP(S554A)

    Plasmid

    #59968

    Purpose

    An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    Prolyl oligopeptidase (PREP Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S554A

    Promoter

    EF1a

    Available Since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-DIO-VGAT-WPRE-pA

    Plasmid

    #39320

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV; R

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

    Plasmid

    #170228

    Purpose

    Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    Map2k7-MAPK9 fusion (MAPK9, Map2k7 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Fusion

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

    Plasmid

    #202617

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    FRT-JEDI-1P-Kv
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0286 Ef1a-Cp36_attB-BFP acceptor

    Plasmid

    #193474

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

    Plasmid

    #117136

    Purpose

    Empty vector encoding BFP-BSD

    Depositor

    Kosuke Yusa

    Insert

    BSD2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid

    #118412

    Purpose

    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

    Depositor

    Nicole Paulk

    Article

    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

    Insert

    Firefly luciferase
    

    Use

    AAV, Luciferase, and Synthetic Biology

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-EF1a-DIO-GRAB_r5-HT1.0

    Plasmid

    #208725

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDH-EF1a-hLMX1A-T2A-copGFP

    Plasmid

    #170444

    Purpose

    Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human LMX1A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Ef1a-Coff/Fon-oScarlet

    Plasmid

    #137138

    Purpose

    Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA

    Plasmid

    #37756

    Purpose

    Expresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    ChETA-TdTomato
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    TdTomato

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

    Plasmid

    #208724

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-EF1A-CD19.8H.28TM.BB.28.3z

    Plasmid

    #200677

    Purpose

    Modular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH-EF1a-tetR-T2A-iRFP

    Plasmid

    #176028

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    iRFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge