We narrowed to 60,833 results for: Ran
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Plasmid#58423Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.DepositorInsertPERK (Eif2ak3 Mouse)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationDeleted amino acids M1 to Y502Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2
Plasmid#236245PurposeCloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoterDepositorInsertmTagBFP2 fused to the plasma membrane targeting sequence CAAX2
UseAAVTagsmTagBFP2PromoterU6 and human Synapsin1Available SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBlueSKMimRNA
Plasmid#102535PurposeTemplate for synthesis of Minos transposase mRNADepositorInsertMinos transposase mRNA
Available SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-134
Plasmid#185925PurposeHimar transposon delivery plasmid that delivers a orange fluorescent mOrange2 protein geneDepositorInsertmOrange2
ExpressionBacterialAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHSS6hsILMi20
Plasmid#102536PurposeHelper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)DepositorInsertMinos transposase
Available SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_QRFV_Hyp
Plasmid#207302PurposeMammalian expression of Quaranfil quaranjavirus Hypothetical proteinDepositorInsertQRFV_Hyp
ExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA CO Tol2 TPase
Plasmid#133032PurposeExpression vector for production of codon optimized Tol2 transposaseDepositorInsertCodon optimized Tol2 Transposase
UseIn vitro expressionMutationCodon OptimizedAvailable SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TfR-OVA
Plasmid#64600PurposeExpression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentationDepositorInsertOvalbumin
TagsTransmembrane domain of Transferrin Receptor (TfR…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
wg186Fluc
Plasmid#177436PurposeIn vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.DepositorInsertFirefly luciferase
UseLuciferase; For in vitro transcriptionTagsNonePromoterT7Available SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pccGFP-MalE-pos-2H01
Plasmid#239760PurposePositive control for liquid MalE complementation assayDepositorInserttransmembrane domain of SEM5A
TagsMBP and ToxRExpressionBacterialMutationChanged Leucine 985 to IsoleucineAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pccGFP-MalE-neg-2H11
Plasmid#239086PurposeNegative control for liquid MalE complementation assayDepositorInserttransmembrane domain of EVA1B
TagsMBP and ToxRExpressionBacterialMutationChanged Leucine 30 to IsoleucineAvailable SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pccGFP-MalE-pos-1F02
Plasmid#239087PurposePositive control for liquid MalE complementation assayDepositorInserttransmembrane domain of AMPE
TagsMBP and ToxRExpressionBacterialMutationdeleted amino acids 19-22, and changed Leucine 35…Available SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pccGFP-MalE-pos-2H07
Plasmid#239088PurposePositive control for liquid MalE complementation assayDepositorInserttransmembrane domain of FND10
TagsMBP and ToxRExpressionBacterialMutationChanged Valine 184 to IsoleucineAvailable SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pccGFP-MalE-neg-2E11
Plasmid#239084PurposeNegative control for liquid MalE complementation assayDepositorInserttransmembrane domain of LRIG3
TagsMBP and ToxRExpressionBacterialMutationChanged Valine 827 to IsoleucineAvailable SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pccGFP-MalE-neg-2F12
Plasmid#239085PurposeNegative control for liquid MalE complementation assayDepositorInserttransmembrane domain of LRIG3
TagsMBP and ToxRExpressionBacterialMutationChanged Valine 815 to IsoleucineAvailable SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_hACE2_HygR
Plasmid#155296PurposeLentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistanceDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag
Plasmid#210021PurposeExpression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.DepositorInsertTransferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Human, Synthetic)
TagsMycTag, CTagExpressionMammalianMutationTransferrin receptor transmembrane domain with Y2…PromoterCMV promoterAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGreen2x35SLUC
Plasmid#108228PurposePlant expression as translational fusions to FLUC with RLUC normalizationDepositorTypeEmpty backboneUseLuciferaseTagsFirefly LuciferaseExpressionPlantPromoter2x35SAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pccGFP-MalE-neg-1E01
Plasmid#239013PurposeNegative control for liquid MalE complementation assayDepositorInserttransmembrane domain of GBGT1
TagsMBP and ToxRExpressionBacterialMutationChanged Leucine 6 to phenylalanine, changed Leuci…Available SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pccGFP-MalE-neg-1E11
Plasmid#239083PurposeNegative control for liquid MalE complementation assayDepositorInsertTransmembrane domain of CEA20
TagsMBP and ToxRExpressionBacterialMutationChanged Alanine 457 to Phenylalanine, and deleted…Available SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW2-MaUPPS-B
Plasmid#203175PurposeYeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferaseDepositorInsertMA_4402
ExpressionYeastPromoterTDH3Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1-MaUPPS-A
Plasmid#203174PurposeYeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferaseDepositorInsertuppS
ExpressionYeastPromoterTDH3Available SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialPromoterPydfJ115Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2-AA007
Plasmid#185750PurposeVector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
Tagsmembrane localization tag Lti6bExpressionPlantAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
L2-AA012
Plasmid#185751PurposeVector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
Tagsmembrane localization tag Lti6bExpressionPlantAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-OEP7-mVenus
Plasmid#213864PurposeBinary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.DepositorInsertchloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CDK9 HA (P#635)
Plasmid#14640DepositorAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…ExpressionPlantPromoterE12-omegaAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.2
Plasmid#89000PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.2 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
EYFP-hDF-deltaTM-pCMV-9
Plasmid#72586Purposehuman dysferlin no transmembrane domain tagged with YFPDepositorInsertdysferlin (DYSF Human)
TagsEYFPExpressionMammalianMutationtransmembrane domain deletedPromoterCMVAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHis6-hDF-deltaTM-pCMV-9
Plasmid#72587Purposehuman dysferlin no transmembrane domain tagged with his tagDepositorInsertdysferlin (DYSF Human)
TagsHisExpressionMammalianMutationtransmembrane domain deletedPromoterCMVAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.1
Plasmid#88994PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.1 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-BLa 1.1
Plasmid#88997PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.DepositorInsertC-BLa 1.1 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mGrn-myc
Plasmid#178944PurposeConstruct for expressing murine progranulin (Grn) in AAVDepositorAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066
Plasmid#175587Purposein vitro transcription of wt RBM8A 3'UTRDepositorInsertRBM8A-3'UTR AluY-Sz WT
UseIn vitro transcriptionPromoterT7Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CFF-EGFP
Plasmid#86061PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.DepositorInsertFibrocystin (Pkhd1 Mouse)
TagsGFPExpressionMammalianMutationcontains luminal and transmembrane domains of CD8…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSV CDK9 HA (P#598)
Plasmid#14638DepositorAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only