We narrowed to 9,269 results for: ef1
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Plasmid#58851PurposeAAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) mannerDepositorInsertArchT-GFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianPromoterEF1aAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-loxP-GRAB_DA2m-loxP
Plasmid#189751PurposeExcludes sensor expression in Cre-positive neuronsDepositorInsertDopamine sensor GRAB_DA2m
UseAAVPromoterEF1aAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a HP1cs-Frb1x PGK Puro
Plasmid#102808PurposeLentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion proteinDepositorInsertHP1cs-Frb1x
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-EF1-IRES-NEO-Control
Plasmid#80757PurposeTransposon ReporterDepositorInsertNeoR (neoR Synthetic)
PromoterEF1-IRES from PB-EF1-IRES-NEOAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA
Plasmid#37087PurposeExpresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibitionDepositorInsertNpHR3.0-EYFP
UseAAV and Cre/Lox; Cre-offTagsEYFPPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-EGFP-T2A-RPL22-1xFlag
Plasmid#170323PurposeExpresses FLAG and EGFP tagged RPL22DepositorAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mTagBFP-T2A-TetOn3G
Plasmid#179888PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsmTagBFPExpressionBacterial and MammalianPromoterEF1aAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]
Plasmid#122102PurposeAAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianPromoterEF1a(1.1kb short version)Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1α-IRES-EGFP-hMCIDAS
Plasmid#149696PurposeExpresses untagged human MCIDAS in mammalian cellsDepositorAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-RPS27A-UTR-PP7-WPRE
Plasmid#198339PurposePB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loopsDepositorInsertRPS27A-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loopsExpressionMammalianPromoterEf1aAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode
Plasmid#170240PurposeBarcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128587PurposeAAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterEF1α (1.1 kb short version)Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO mutant ITR
Plasmid#67905PurposePiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutationDepositorInsertNeoR (neoR Synthetic)
ExpressionMammalianAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-hPOT1_IRES-blast
Plasmid#166411Purposeexpress hPOT1 in mammalian cellsDepositorAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-mCherry-T2A-RPL22-1xV5
Plasmid#170324PurposeExpresses V5 and mCherry tagged RPL22DepositorAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-VGAT-WPRE-pA
Plasmid#39320DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV; RAvailable SinceOct. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast
Plasmid#71217PurposedCas9 fused to human DNMT3B catalytic domainDepositorInsertdSpCas9-DNMT3B catalytic domain
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-mRuby3-2A-dCre
Plasmid#203845PurposeFlp-dependent enzymatically inactive Cre and mRuby3 expressionDepositorInsertdCre
UseAAVTagsmRuby3-2AMutationEnzymatically inactive CreAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pb459-mU6-sgRNA-EF1a-PuroR
Plasmid#195507Purposepiggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sitesDepositorInsertPuromycin
UseCRISPR; PiggybacExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQLL-Tc1-8B_DR[EF1α-copGFP]
Plasmid#218327PurposeDonor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassetteDepositorInsertcopGFP mammalian expression cassette
ExpressionMammalianAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD
Plasmid#117136PurposeEmpty vector encoding BFP-BSDDepositorInsertBSD2A
UseLentiviralExpressionMammalianMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-hLMX1A-T2A-copGFP
Plasmid#170444PurposeLentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.DepositorInserthuman LMX1A
UseLentiviralPromoterEF1aAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-oScarlet
Plasmid#137138PurposeIntersectional viral expression of oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationE95DPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC446 - pAAV EF1a hPREP(S554A)
Plasmid#59968PurposeAn AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.DepositorAvailable SinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA
Plasmid#37756PurposeExpresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-offTagsTdTomatoPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE
Plasmid#202617PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependentDepositorInsertFRT-JEDI-1P-Kv
UseAAV; Flp/frtExpressionMammalianPromoterEF1aAvailable SinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_r5-HT1.0
Plasmid#208725PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinaseDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVPromoterEF1aAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode
Plasmid#170228PurposeBarcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorUseLentiviralTagsFLAGMutationFusionPromoterEF1aAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-S1977P
Plasmid#64876Purposelentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationS1977P, mimicking the chaos1 mutationPromoterEF1alphaAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-mouse-full-length-CHMP3-HA
Plasmid#154176Purposeexpresses mouse CHMP3 in mammalian cellsDepositorAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.BB.28.3z
Plasmid#200677PurposeModular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
C413-E15: attB4-EF1p>-attB1r
Plasmid#162920PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman EF1alpha promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-iRFP
Plasmid#176028Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720DepositorInsertstetR
iRFP
UseLentiviralAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA
Plasmid#37086PurposeExpresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-YFP
UseAAV and Cre/Lox; Cre-offTagsEYFPPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAIO-Ef1a-PE2-GFP:KCNQ2-C201R
Plasmid#185060PurposeEf1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445DepositorInsertU6:pegRNA:scaffold:PBS+RT template
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT3.0mut
Plasmid#208724PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseAAVPromoterEF1aAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a HP1cs-Frb2x PGK Puro
Plasmid#102809PurposeLentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion proteinDepositorInsertHP1cs-Frb2x
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-GFP-miRE_shRen-Blast
Plasmid#135682PurposeConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-NCreIntN-EF1a-mTagBFP2
Plasmid#160507PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianPromoterCAGAvailable SinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mCherry-WPRE
Plasmid#202620PurposeDouble floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertmCherry
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only