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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 7181 - 7200 of 8782 results

  11. MTK0_001

    Plasmid
    #123924
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    TU-sgRNA - L1/RE
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123924-plasmid-map-sequence-id-250078

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  12. pCALNL_chr13_102010574-102010650

    Plasmid
    #81213
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81213-plasmid-map-sequence-id-163268

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  13. MTK234_055

    Plasmid
    #123920
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123920-plasmid-map-sequence-id-250070

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  14. pCALNL_Ch12_62418577-62418652

    Plasmid
    #81212
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81212-plasmid-map-sequence-id-163264

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  15. pOCC215

    Plasmid
    #118879
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    Tags
    Dendra2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118879-plasmid-map-sequence-id-232264

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  16. pOCC207

    Plasmid
    #118869
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mCherry
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118869-plasmid-map-sequence-id-232318

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  17. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126054-plasmid-map-sequence-id-254921

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  18. pMiNGo11-1

    Plasmid
    #126056
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126056-plasmid-map-sequence-id-246974

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Showing: 7181 - 7200 of 8782 results