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pACT5FVMF(f)

Plasmid

#110549

Purpose

Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

Depositor

David Russell

Article

Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

Insert

EGFP, FV LTRs

Use

Qpcr control for titering foamy virus

Promoter

promoter: MLV LTR

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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shPBRM1#131-Tet-pLKO-puro

Plasmid

#107400

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSLC-238

Plasmid

#73192

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-chpB (chpB )

Expression

Bacterial

Promoter

PrhaB

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2189

Plasmid

#67532

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pGME

Plasmid

#71252

Purpose

luciferase reporter with GM-CSF promoter + 717 bp Bgl II GM-CSF enhancer

Depositor

Peter Cockerill

Article

Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

Insert

GM-CSF enhancer (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GM-CSF

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pES4/4a

Plasmid

#69590

Purpose

TCR a chain cDNA in pES4 transgenic construct for creating OT-II mice

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR alpha (Tcra Mouse)

Expression

Mammalian

Promoter

H-2Kb

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
pMDC32B-OsMIR390-B/c

Plasmid

#61467

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Expression

Plant

Mutation

O. sativa MIR390 precursor sequence including a c…

Promoter

2x35S

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBI101-pGC1(D1)-GUS

Plasmid

#49513

Purpose

Expresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plant

Depositor

Julian Schroeder

Article

Yang et al Plant Methods. 2008 Feb 19;4:6. doi: 10.

Insert

Promoter of GC1(D1) (AT1G22690 Mustard Weed)

Use

Plant expression

Tags

beta-glucuronidase

Promoter

GC1 (At1g22690), 1141 bp upstream of the transcri…

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pMT3-FLAG-KLF3

Plasmid

#49102

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Himeda et al Mol Cell Biol. 2010 Jul;30(14):3430-43.

Insert

Krüppel-Like Factor 3(KLF3)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-C9orf114-flag

Plasmid

#70647

Purpose

Expresses C9orf114 with FLAG-tag epitope.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C9orf114 (SPOUT1 Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-poly-RGD

Plasmid

#67002

Purpose

Expresses malE-poly-RGD in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly-RGD

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-ELP[V5A2G3-60]

Plasmid

#67009

Purpose

Expresses malE-ELP[V5A2G3-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[V5A2G3-60]

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-C9orf114-gfp

Plasmid

#70266

Purpose

Expresses C9orf114 in mammalian cells with GFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C9orf114 (SPOUT1 )

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-C3orf17-gfp

Plasmid

#70265

Purpose

Expresses C3orf17 in mammalian cells with GFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C3orf17 (NEPRO Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only
pT-TK

Plasmid

#31549

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

LoxP

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pINS-Neo

Plasmid

#31390

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

aph

Use

Cre/Lox

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pDONRHYG

Plasmid

#29636

Depositor

David_L Andrews, Scott Gold

Article

Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

Type

Empty backbone

Use

Gateway Cloning

Available Since

Oct. 5, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pMyt1a

Plasmid

#22695

Depositor

Lynn Hudson

Article

Kim et al Mol Cell Biol. 1992 Dec . 12(12):5632-9.

Insert

Human Myelin Transcription Factor 1 (MYT1 Human)

Use

Cloning vector

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only
M24 Nac>Nac

Plasmid

#17174

Depositor

Randall Moon

Article

Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

Insert

nacre promoter driving nacre cDNA (mitfa Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
PCD FL6X

Plasmid

#12566

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

Four CXCR4 imperfect binding sites (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos Y345A

Plasmid

#8970

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Y345A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos DD T325A T331A

Plasmid

#8973

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D, T325A, T331A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pTHSSe_52

Plasmid

#109250

Purpose

ColE1 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_37

Plasmid

#109237

Purpose

PTac driving GFP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PTac

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_40

Plasmid

#109239

Purpose

PTac driving TALEsp2

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

TALEsp2

Expression

Bacterial

Promoter

PTac

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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M3926 leu2::KanMX3 Disruptor Converter

Plasmid

#51680

Purpose

Yeast marker swap plasmid for converting LEU2 to KanMX3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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myc-NLRC5 deltaDD

Plasmid

#37511

Depositor

Thomas Kufer

Article

Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

Insert

NLRC5 (NLRC5 Human)

Tags

myc

Expression

Mammalian

Mutation

lacking the N-terminal domain (deletion: aa 1-134)

Promoter

CMV

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pRiboS EcoHind

Plasmid

#36254

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pRibo EcoHind

Plasmid

#36252

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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TALEN1258

Plasmid

#32281

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gria3a (gria3a Zebrafish)

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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