We narrowed to 22,830 results for: Ide
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Plasmid#53153PurposeExpresses full-length CD58 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD58 (CD58 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-aZEA
Plasmid#53286PurposeContains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coliDepositorAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-Myr-FLAG-p110α-DM
Plasmid#55713Purposeeukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227ADepositorInsertp110 alpha (Pik3ca Mouse)
Tagsmyristoylation tag (Myr-FLAG)ExpressionMammalianMutationT207D, K227APromoterT7Available SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC148-pCR8-dCas9VP96
Plasmid#48220PurposedCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPR and Gateway CloningTagsHA Tag and VP96MutationD10A;H840AAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
Drosophila_sgRNA_group_2
Pooled Library#134583PurposeTargets Drosophila orthologs of vertebrate genes that have undergone expansion in the vertebrate lineDepositorExpressionInsectSpeciesDrosophila melanogasterUseCRISPRAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Drosophila_sgRNA_group_1
Pooled Library#134582PurposeTargets kinases, phosphatases, and Drosophila orthologs of drug targetsDepositorExpressionInsectSpeciesDrosophila melanogasterUseCRISPRAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA
Plasmid#218710PurposeAAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoterDepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_D2A_tdTomato
Plasmid#184045PurposeExpresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)DepositorInsertsEnhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence
ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H CC editing reporter
Plasmid#198885PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralExpressionMammalianMutationY93H, Gly92(GGT-to-GGC)PromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EFNB2 (WT)
Plasmid#200975PurposeMammalian expression plasmid for wild type, myc-tagged EFNB2DepositorInsertEFNB2 (EFNB2 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM049 His-TEV-Cas9 triNLS 2C
Plasmid#196245PurposeExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_1G11F2-3 light chain
Plasmid#198254Purposerecombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3DepositorInsertlight chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)
TagsIL10 signal peptideExpressionMammalianPromoterCMVAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-APEX2-Ires-eGFP-PAC
Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PTBP1-Halo
Plasmid#175302PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
4LNDDE488Q-pCDNA3.1(+)
Plasmid#172228PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
Tags6xHis and FlagExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT D554N
Plasmid#154279PurposeBacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationAspartate 554 changed to Asparagine- bacterial co…Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS*11_4enh
Plasmid#149423PurposePlasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).DepositorInsertsrbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer
TagsT120>AExpressionBacterialAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS-N-HA (murine)
Plasmid#130916PurposeExpresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection markerDepositorInsertmurine cGAS N terminus
TagsHAExpressionMammalianMutationmurine cGAS N terminus (aa 1-147)PromoterCMVAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W
Plasmid#105040PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-ZM197M-gp160
Plasmid#123226PurposeMammalian expression plasmid for Env from the ZM197M HIV-1 isolateDepositorInsertHIV-1 (ZM197M) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only