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pEM705 Gst (Cys)

Plasmid

#109376

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD2

Plasmid

#102976

Purpose

Suppression of SOD2

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD2 (SOD2 Human)

Use

Lentiviral and RNAi

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pACS131

Plasmid

#204596

Purpose

Histone H3.1K27M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 K27M (H3C1 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEF6a-BCCP-PupE

Plasmid

#113399

Purpose

Expresses BCCP-PupE in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPerFLEX

Plasmid

#217569

Purpose

Bacterial expression of HyPerFLEX

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX

Tags

6xHis

Expression

Bacterial

Promoter

T5

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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UBC1cer120G

Plasmid

#161017

Purpose

ubiquitously weakly expressed 120-meric GFP nanocage

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

120meric GFP nanocage

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEntr-GATA4flbio

Plasmid

#32937

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

GATA4 (Gata4 Mouse)

Use

Gateway

Tags

flag-bio

Promoter

none

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pUASTattB-degradALFA

Plasmid

#219663

Purpose

Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

degradALFA

Expression

Insect

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-SLC35A1-GFP

Plasmid

#186281

Purpose

transient expression of C-terminal GFP tagged SLC35A1 isoform a in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A1 (SLC35A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBI-p53 wt/EGFP

Plasmid

#16543

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pCN34e-ktrA-yfp

Plasmid

#227640

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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sgPPP1R2

Plasmid

#227391

Purpose

Adhesion module, guide RNA recognizes PPP1R2

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

guide RNA targeting PPP1R2

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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HLA_B5101

Plasmid

#165011

Purpose

Expression of HLA-B*51:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-B*51:01 (HLA-B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
MiniCoopR U6:gRNA, mitfa:Cas9

Plasmid

#118840

Purpose

Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC-ARS-stacked-NeoR-mNeonGreen

Plasmid

#101249

Purpose

Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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p3998 pGEX HHR23A

Plasmid

#10864

Depositor

Peter Howley

Article

Kumar et al J Biol Chem. 1999 Jun 25. 274(26):18785-

Insert

HHR23A (RAD23A Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
TOPO mCherry

Plasmid

#68490

Purpose

TOPO construct expressing mCherry for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mCherry

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pmNFL

Plasmid

#83127

Purpose

Mouse neurofilament protein L cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFL (Nefl Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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