165,545 results

1. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

   Plasmid

   #228363

   Purpose

   mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCAR_sgRNA_puro-mKate-lox2272

   Plasmid

   #162076

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EML4-ALK variant 3a

   Plasmid

   #183829

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUGW-ER-TurboID

   Plasmid

   #200965

   Purpose

   3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   ER-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b_Bst-LF

   Plasmid

   #153313

   Purpose

   Bacterial expression of the Bst Large Fragment

   Depositor

   Philippa Marrack, Drew Endy

   Insert

   Bst Large Fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. mEGFP-H4-23

   Plasmid

   #56463

   Purpose

   Localization: Nucleus/Histones, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Insert

   H4 (HIST1H4J Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 23, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. NL4-3 mCherry Luciferase

   Plasmid

   #44965

   Purpose

   HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.

   Depositor

   Warner Greene

   Article

   Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371

   Insert

   mCherry, Luciferase, IRES
   

   Use

   Lentiviral

   Tags

   Luciferase, IRES and mCherry with D212G

   Expression

   Mutation

   S34C mutation in Nef

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExpreS2-1-C3-10H-SIII

   Plasmid

   #175444

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   3C-10His-TwinStrep and BiP Secretion Peptide

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. opto-E-cad GFP

   Plasmid

   #203327

   Purpose

   Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

   Depositor

   Seraphine Wegner

   Article

   Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

   Insert

   Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Addition of Aslov2 domain at T133 of human E-cadh…

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-DIO-TVA-oG-mCherry

    Plasmid

    #166602

    Purpose

    Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    TVA-oG-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-neo-PP1-Alpha

    Plasmid

    #171267

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pANAP

    Plasmid

    #48696

    Purpose

    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

    Depositor

    Peter Schultz

    Article

    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

    Inserts

    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutant E.coli leucyl synthetase specific for Anap…

    Promoter

    CMV and human H1 promoter

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDARMO_HUWE1_3xFLAG

    Plasmid

    #187155

    Purpose

    mammalian cell expression of HUWE1-3xFLAG

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPRv2FE-ABE8e-SpRY

    Plasmid

    #213008

    Purpose

    A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO (pEHA1339)

    Plasmid

    #209088

    Purpose

    Backbone plasmid for Tet Responsive transgene expression, FRT integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8e-N108Q

    Plasmid

    #194157

    Purpose

    Mammalian expression of ABE8e-N108Q

    Depositor

    Dali Li

    Article

    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

    Insert

    TadA-8e (N108Q)-nSpCas9
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. peYFP-C1-mTOR

    Plasmid

    #73384

    Purpose

    Expresses YFP tagged mTOR

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pErCas12a EZ Clone

    Plasmid

    #132641

    Purpose

    Expresses ErCas12a and an sgRNA to target a region of interest

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    AAV and CRISPR

    Tags

    3x HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q926R (Please see depositor comments) and BsaI si…

    Promoter

    CMV Promoter

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRES2-EGFP-SHP2WT

    Plasmid

    #12283

    Depositor

    Anton Bennett

    Article

    Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

    Insert

    SHP-2WT (PTPN11 Human)
    

    Use

    Tags

    Myc (not canonical: KLISEEDL)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-ZDHHC17

    Plasmid

    #231665

    Purpose

    Expresses human ZDHHC17protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC17 (ZDHHC17 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDH-EF1-IRES eGFP

    Plasmid

    #128059

    Purpose

    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJL-TRBO-G

    Plasmid

    #80083

    Purpose

    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSO2002

    Plasmid

    #25842

    Depositor

    Aziz Sancar

    Article

    Ozgur et al Biochemistry. 2003 Mar 18. 42(10):2926-3

    Insert

    human Cryptochrome 2 (CRY2 Human)
    

    Use

    Tags

    flag, his, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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24. AAV-CAG-jGCaMP8s-WPRE

    Plasmid

    #179256

    Purpose

    AAV-mediated expression of GCaMP8s under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pENTR4-HaloTag (w876-1)

    Plasmid

    #29644

    Depositor

    Eric Campeau

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. pRSET-MagLOV

    Plasmid

    #219957

    Purpose

    Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

    Depositor

    Andrew York

    Article

    Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

    Insert

    MagLOV
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    AsLOV2 C450P L496V Q513K G528K D540M

    Promoter

    T7

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pWZL-Neo-Myr-Flag-DEST

    Plasmid

    #15300

    Purpose

    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

    Depositor

    Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSBbi-BP

    Plasmid

    #60512

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. mEmerald(luminal)-canxTM

    Plasmid

    #186961

    Purpose

    Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) with ER signal pe…

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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