285,398 results

1. Lyn-tailed mCherry-SEpHluorin

   Plasmid

   #32002

   Depositor

   Sergio Grinstein

   Article

   Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

   Insert

   Membrane-targeting sequence of Lyn kinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. Yamamoto Lab TALEN Accessory Pack

   Plasmid Kit

   #1000000030

   Purpose

   Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

   Depositor

   Takashi Yamamoto

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFPmAID

   Plasmid

   #174213

   Purpose

   GFP::mAID donor backbone

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFPmAID

   Plasmid

   #174213

   Purpose

   GFP::mAID donor backbone

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mito-EcSTH

   Plasmid

   #211921

   Purpose

   Mitochondrial localization of EcSTH

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   mito-EcSTH
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRGEB31

   Plasmid

   #51295

   Purpose

   Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 and dual 35S promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRGEB31

   Plasmid

   #51295

   Purpose

   Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 and dual 35S promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ404-DogCatcher-sfGFP

   Plasmid

   #171930

   Purpose

   Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher-sfGFP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJ404-DogCatcher-sfGFP

    Plasmid

    #171930

    Purpose

    Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher-sfGFP
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF3195

    Plasmid

    #144671

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT1 (ONECUT1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF3195

    Plasmid

    #144671

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT1 (ONECUT1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWN_U6-B2M-miniGag

    Plasmid

    #228957

    Purpose

    miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM553

    Plasmid

    #68543

    Purpose

    NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

    Depositor

    Christopher Voigt, Timothy Lu

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM553

    Plasmid

    #68543

    Purpose

    NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRE-UBC-HLA-E SCT-T2A-GFP

    Plasmid

    #205461

    Purpose

    lentiviral plasmid for expression of HLA-E SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    HLA-E SCT (HLA-E Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-hACE2-LgBiT

    Plasmid

    #215837

    Purpose

    Expresses hACE2 receptor with LgBiT tag in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    hACE2-LgBiT (ACE2 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. miRFP670nano-pcDNA

    Plasmid

    #127443

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. miRFP670nano-pcDNA

    Plasmid

    #127443

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N1_hTRIM32

    Plasmid

    #69541

    Purpose

    Expresses human TRIM32 fused to c-terminal EGFP

    Depositor

    Martin Dorf

    Article

    Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

    Insert

    tripartite motif containing 32 (TRIM32 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-N1_hTRIM32

    Plasmid

    #69541

    Purpose

    Expresses human TRIM32 fused to c-terminal EGFP

    Depositor

    Martin Dorf

    Article

    Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

    Insert

    tripartite motif containing 32 (TRIM32 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Human Kinase Domain Constructs for Automated Bacterial Expression

    Plasmid Kit

    #1000000094

    Purpose

    68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expression

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJFRC19-13XLexAop2-IVS-myr::GFP

    Plasmid

    #26224

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    myr::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJFRC19-13XLexAop2-IVS-myr::GFP

    Plasmid

    #26224

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    myr::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2_IRES2_EGFP_KCNQ2 var4

    Plasmid

    #173154

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Simian CMV IE94

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2_IRES2_EGFP_KCNQ2 var4

    Plasmid

    #173154

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Simian CMV IE94

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge