322,191 results

1. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Neil Fairweather, Robert Fagan

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Neil Fairweather, Robert Fagan

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBTK622

   Plasmid

   #180592

   Purpose

   tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

   Depositor

   Jeffrey Barrick

   Article

   Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

   Inserts

   tdk
   kanR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK622

   Plasmid

   #180592

   Purpose

   tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

   Depositor

   Jeffrey Barrick

   Article

   Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

   Inserts

   tdk
   kanR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. phU1-PspCas13b-non-targeting (pEZ36)

   Plasmid

   #229053

   Purpose

   Cloning backbone for PspCas13b crRNA (Human U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. phU1-PspCas13b-non-targeting (pEZ36)

   Plasmid

   #229053

   Purpose

   Cloning backbone for PspCas13b crRNA (Human U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNL [NlucP/ISRE/Hygro] Vector

   Plasmid

   #236834

   Purpose

   Express ISRE response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   ISRE
   

   Use

   Luciferase

   Tags

   NanoLuc (R)

   Expression

   Mutation

   None

   Promoter

   minP

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET303_sumocodo_GFP_codoopti

   Plasmid

   #210393

   Purpose

   Express SUMO-GFP in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Tags

   SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pET303_sumocodo_GFP_codoopti

   Plasmid

   #210393

   Purpose

   Express SUMO-GFP in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Tags

   SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. AttB_mCherry

    Plasmid

    #171598

    Purpose

    Strong mCherry fluorescence from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Insert

    mCherry
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AttB_mCherry

    Plasmid

    #171598

    Purpose

    Strong mCherry fluorescence from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Insert

    mCherry
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKL2351

    Plasmid

    #199721

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKL2351

    Plasmid

    #199721

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #232929

    Purpose

    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    OsTir1(F74G)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 74 to Glycine

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #232929

    Purpose

    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    OsTir1(F74G)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 74 to Glycine

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT-IMD-V5-His

    Plasmid

    #63748

    Purpose

    Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    immune deficiency (imd Fly)
    

    Use

    Tags

    V5-6XHis

    Expression

    Insect

    Mutation

    *see comment below

    Promoter

    pMT

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMT-IMD-V5-His

    Plasmid

    #63748

    Purpose

    Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    immune deficiency (imd Fly)
    

    Use

    Tags

    V5-6XHis

    Expression

    Insect

    Mutation

    *see comment below

    Promoter

    pMT

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCI HA NEDD4

    Plasmid

    #27002

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4 (NEDD4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI HA NEDD4

    Plasmid

    #27002

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4 (NEDD4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-MbPylRS(DiZPK)

    Plasmid

    #91706

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

    Inserts

    pylS
    pylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Promoter

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge