167,642 results

1. PIP2-GFP(ST) sensor

   Plasmid

   #170356

   Purpose

   GFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

   Depositor

   Kees Jalink

   Article

   van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

   Insert

   GFP(ST)-PH (PLCD1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSW002-PpsbA_mTurquoise2

   Plasmid

   #108237

   Purpose

   Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   PsbA promoter - mTurquoise2
   

   Expression

   Bacterial

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1_puro_shNRF2-1

   Plasmid

   #230086

   Purpose

   shRNA silencing human NRF2 gene

   Depositor

   Xingguo Zhu

   Article

   Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

   Insert

   Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK5785

   Plasmid

   #219753

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnLuz_v4 - tAOX
   

   Use

   Luciferase

   Expression

   Yeast

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Flag-Tet3

   Plasmid

   #60940

   Purpose

   Expresses Tet3 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 3
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-H2BmGreenLantern

   Plasmid

   #177332

   Purpose

   To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

   Depositor

   Gregory Petsko, Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   H2B (Hist1h2bq Rat)
   

   Use

   AAV

   Tags

   mGreenLantern

   Expression

   Mammalian

   Mutation

   This fusion protein was optimized to the Human co…

   Promoter

   CAG

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIPZ shSTING-Hsa

   Plasmid

   #128158

   Purpose

   Doxycyclin inducible shRNA knockdown of human STING gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shSTING Hsa (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 CARD8-TEV-GFP-FLAG

   Plasmid

   #169991

   Purpose

   Mammalian purification construct for CARD8

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   TEV-GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO-TRE-AFOS

   Plasmid

   #197079

   Purpose

   A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

   Depositor

   Elaine Fuchs

   Article

   Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

   Insert

   AFOS (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and T7

   Promoter

   TRE

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TBG-ER-TurboID (AAV8)

    Viral Prep

    #149415-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-TBG-ER-TurboID (#149415). In addition to the viral particles, you will also receive purified pAAV-TBG-ER-TurboID plasmid DNA. TBG-driven expression of ER-localized TurboID (with HA and V5 tag). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Promoter

    TBG

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

11. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209730

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PM-RA

    Plasmid

    #209878

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNP566

    Plasmid

    #222724

    Purpose

    pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-5'+3' MS2-CircRNA

    Plasmid

    #213752

    Purpose

    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Golgi-mEGFP

    Plasmid

    #182877

    Purpose

    Localization: Gal-T - Golgi Complex

    Depositor

    Salvatore Chiantia

    Article

    Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

    Insert

    Golgi-mEGFP
    

    Expression

    Mammalian

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRU43

    Plasmid

    #167665

    Purpose

    ENTRY vector for gRNA3 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-hDAT

    Plasmid

    #32810

    Depositor

    Susan Amara

    Insert

    DAT (SLC6A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a MDHC_YEAST

    Plasmid

    #204232

    Purpose

    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC_YEAST (MDH2 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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