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HIV-1 LTR-gfp

Plasmid

#115809

Purpose

HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp and ngfr

Use

Lentiviral

Promoter

HIV1 LTR

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET22HT-PP7delFG

Plasmid

#31863

Depositor

Robert Singer

Publication

Chao et al Nat Struct Mol Biol. 2008 Jan;15(1):103-

Insert

PP7delFG Coat Protein

Tags

His6-tag and TEV site

Mutation

deleted FG loop

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mNeonGreen-Rab7

Plasmid

#129603

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Tagged-Cap

Plasmid

#35572

Depositor

Marian Waterman

Publication

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Type

Empty backbone

Use

Luciferase

Tags

Ms2 Stem Loop

Expression

Mammalian

Promoter

SV40

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Publication

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJZC43

Plasmid

#66565

Purpose

sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2XPP7
PCP-VP64 IRES mCherry

Expression

Mammalian

Promoter

CMV and U6

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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YFP-Parkin-IRES-zeo

Plasmid

#61728

Purpose

Expression of human Parkin fused to eYFP

Depositor

Douglas Green, Stephen Tait

Publication

Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

Insert

Parkin (PRKN Human)

Tags

eYFP

Expression

Mammalian

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA TrkB

Plasmid

#24088

Depositor

Moses Chao

Publication

Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

Insert

TrkB (Ntrk2 Rat)

Expression

Mammalian

Mutation

R133I

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFastBac1 Flag BAF155 b

Plasmid

#1954

Depositor

Robert Kingston

Publication

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BAF155 (SMARCC1 Human)

Tags

flag

Expression

Insect

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-PE6f

Plasmid

#207856

Purpose

Mammalian expression of PE6f prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6f

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-PE6g

Plasmid

#207857

Purpose

Mammalian expression of PE6g prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6g

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-C1X-Nup93

Plasmid

#87336

Purpose

To express human Nup93 in mammalian cells

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hRIP1 HA GFP wt

Plasmid

#41396

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP1 (RIPK1 Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

E96G

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

Pooled library

#84003

Purpose

Mouse CRISPRa Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Publication

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
hRIP3 GFP RHIM mut

Plasmid

#41385

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant (AAAA-459-462)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin G13R

Plasmid

#60615

Purpose

Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

Depositor

Primal de Lanerolle

Publication

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

G13R

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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