We narrowed to 25,229 results for: nes

1. pGFP-nE-mHP1alpha-delCTE

   Plasmid

   #181896

   Purpose

   Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   nE-mHP1alpha-delCTE (Cbx5 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

   Promoter

   CMV

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPHR-mCherry-nE-mHP1alpha

   Plasmid

   #181890

   Purpose

   Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-nE-mHP1alpha (Cbx5 Mustard Weed, Mouse)
   

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E

   Promoter

   CMV

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. C. elegans miniMos transgenesis kit

   Plasmid Kit

   #1000000031

   Purpose

   29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.

   Depositor

   Erik Jorgensen

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pPyCAG-NE-mKate2-IRES-Hph

   Plasmid

   #183616

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

   Depositor

   Sally Lowell

   Article

   Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

   Insert

   mKate2
   

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPyCAG-NE-EGFP-IRES-Pac

   Plasmid

   #183617

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

   Insert

   EGFP
   

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHR-mCherry-nE-mHP1alpha-delCTE

   Plasmid

   #181891

   Purpose

   Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mustard Weed, Mouse)
   

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

   Promoter

   CMV

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bcr DH domain NE/AA pGEX

   Plasmid

   #38180

   Depositor

   Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   BCR DH domain N689A/E690A (BCR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   BCR DH domain N689A/E690A

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

8. Abr DH domain NE/AA pGEX

   Plasmid

   #38182

   Depositor

   Nora Heisterkamp

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   ABR DH domain N282AE283A (ABR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   ABR DH domain with N282A and E283A mutations

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

9. DHFR Saturation Mutagenesis Library - WT TYMS

   Pooled Library

   #1000000194

   Depositor

   Kimberly Reynolds

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM)

    Pooled Library

    #198050

    Purpose

    By supplying an AAV cap gene of interest in trans, researchers can use this library to investigate the effects of rep mutations on production of a wide range of AAV capsid serotypes

    Depositor

    George Church

    Article

    Jain et al Elife. 2024 Mar 27;12:RP87730. doi: 10.7

    Expression

    Mammalian

    Use

    AAV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

11. DHFR Saturation Mutagenesis Library - Q33S TYMS

    Pooled Library

    #1000000196

    Depositor

    Kimberly Reynolds

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

12. DHFR Saturation Mutagenesis Library - R166Q TYMS

    Pooled Library

    #1000000195

    Depositor

    Kimberly Reynolds

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pRep-Cap Scanning Saturation Mutagenesis (SSM) pooled library

    Pooled Library

    #199601

    Purpose

    pRep-Cap SSM library enables VP1, VP2, and VP3 expression under the control of the endogenous p40 promoter

    Depositor

    George Church

    Article

    Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

    Expression

    Mammalian

    Species

    Aav2

    Use

    AAV

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

14. CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines

    Pooled Library

    #86538

    Depositor

    Jonathan Weissman

    Article

    Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

15. Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

    Pooled Library

    #136479

    Purpose

    Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

    Depositor

    Jonathan Weissman

    Article

    Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

16. CRISPRi non-coding library (CRiNCL) - Common to all 5 cancer cell lines

    Pooled Library

    #86539

    Depositor

    Jonathan Weissman

    Article

    Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

17. Large-scale mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

    Pooled Library

    #136478

    Purpose

    Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

    Depositor

    Jonathan Weissman

    Article

    Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-hsyn-NE2h (AAV5)

    Viral Prep

    #208687-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hsyn-NE2h (#208687). In addition to the viral particles, you will also receive purified pAAV-hsyn-NE2h plasmid DNA. Synapsin-driven expression of genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

    Promoter

    Syn

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

19. lentiCas9-Blast (Lentiviral Prep)

    Viral Prep

    #52962-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Promoter

    EFS-NS

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pLV_hEF1a_rtTA3

    Plasmid

    #61472

    Purpose

    3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Insert

    rtTA3
    

    Use

    Lentiviral

    Promoter

    hEF1a

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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