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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,063 results for: ARIA;

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Showing: 7481 - 7500 of 12063 results

  1. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  8. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Article
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    102562-plasmid-map-sequence-id-203161

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  11. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid
    #212022
    Purpose
    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    GianCreg (GOLGB1 Human)
    Tags
    td5StayGold(c4)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212022-plasmid-map-sequence-id-419221

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  17. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid
    #139994
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139994-plasmid-map-sequence-id-275519

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Showing: 7481 - 7500 of 12063 results