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CMV-ShadowY-kCAAX

Plasmid

#115564

Purpose

dark ITRACK acceptor construct for dual color imaging

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

ShadowY with KRas membrane targeting domain

Tags

ShadowY

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pROSA26-FlpERT2

Plasmid

#149438

Purpose

ROSA26 mouse targeting vector with tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-p27Luc

Plasmid

#23047

Depositor

William Kaelin

Article

Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Insert

p27 (CDKN1B Human)

Tags

Luciferase

Expression

Mammalian

Mutation

wt cDNA

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pQTEV-SNX3

Plasmid

#31573

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

sorting nexin 3 (SNX3 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.mCherry-GCaMP7s

Plasmid

#172385

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized mCherry + GCaMP7s

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pQTEV-PPP1R1A

Plasmid

#31331

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

protein phosphatase 1 (PPP1R1AP1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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RhGC(BE)_pGEM

Plasmid

#85469

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Peter Hegemann

Article

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Insert

rhodopsin-guanylyl cyclase

Expression

Mammalian

Promoter

T7 Promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-FAID

Plasmid

#127885

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID (PPP2CA Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-mCherry

Plasmid

#164426

Purpose

AAV plasmid expressing mCherry in photoreceptors

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pQTEV-CIP29

Plasmid

#34822

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cytokine induced protein 29 kDa (SARNP Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pPMVAu5 (R)

Plasmid

#98296

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ5135: pCAG-BFP-GuideArray

Plasmid

#210606

Purpose

Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

pCAG-BFP-GuideArray

Use

CRISPR

Promoter

CAG

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.mCherry-FlpO

Plasmid

#172378

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized mCherry + FlpO

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pUCBB-ctH6-eGFP

Plasmid

#32562

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pGUS (GB0208)

Plasmid

#68203

Purpose

Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

GUS coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDN42

Plasmid

#61345

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS10 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD4-Y429H

Plasmid

#33041

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Mutation

Y429H

Available Since

March 28, 2012

Availability

Academic Institutions and Nonprofits only
pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

Plasmid

#205261

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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saposin B^W

Plasmid

#161759

Purpose

Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

Depositor

Sam Gellman

Article

Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

Insert

saposin B R38_W

Tags

His-tag

Expression

Bacterial

Mutation

changed Arginine 38 to Tryptophan

Promoter

T7

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJ23119-sgRNA

Plasmid

#113654

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter J23119 and sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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