We narrowed to 8,732 results for: Set

1. pET-30a(+)-Syk-tSH2-PM

   Plasmid

   #111271

   Purpose

   express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
   

   Expression

   Bacterial

   Mutation

   Y130E mutation to mimic phosphorylation

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH231-EF1-BLST-Cas9-VPR

   Plasmid

   #115147

   Purpose

   Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   BlastR-P2A-Cas9-VPR
   

   Use

   CRISPR

   Tags

   SV40NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Arabidopsis thaliana Atm3 d80 pET21a+

   Plasmid

   #173045

   Purpose

   Expresses AtAtm3 in E.coli

   Depositor

   Douglas Rees

   Article

   Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

   Insert

   Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   with N-terminal 80 amino acids deletion

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMDC32B-OsMIR390-B/c

   Plasmid

   #61467

   Purpose

   Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

   Depositor

   James Carrington

   Article

   Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

   Insert

   OsMIR390-B/c
   

   Expression

   Plant

   Mutation

   O. sativa MIR390 precursor sequence including a c…

   Promoter

   2x35S

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBK.014

   Plasmid

   #187215

   Purpose

   Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7/Lac

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC120

   Plasmid

   #118892

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and mGFP, HIS6, c…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pv6_dPC_Chromo_TAF3_Phd

   Plasmid

   #179403

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   dPC_Chromo - TAF3_Phd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179070

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondria

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-preCOX4-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179052

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleus

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-SRM1-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xG1 PylT sfGFP 150TAG

    Plasmid

    #154767

    Purpose

    plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

    Plasmid

    #179035

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Neon(gfp)-Link-SCS2tmh
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA0055

    Plasmid

    #131774

    Purpose

    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMDC123SB-OsMIR390-B/c

    Plasmid

    #61466

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Expression

    Plant

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    2x35S

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUPD2 HDV (GB1444)

    Plasmid

    #160576

    Purpose

    Hepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    HDV
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCXLE-dCas9VPP300-T2A-GFP-shP53

    Plasmid

    #102896

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPP300-T2A-GFP-shP53
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

    Plasmid

    #83461

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, R4113A

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AID-BFP-loxP_myo2_neoR

    Plasmid

    #194054

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Tags

    bfp

    Expression

    Worm

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid

    #157981

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sox17(2A-phiC31o)

    Plasmid

    #140762

    Purpose

    Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    phiC31o
    

    Use

    Mouse Targeting

    Promoter

    Endogenous Sox17

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. ins:BB1.0L; cryaa:RFP

    Plasmid

    #110283

    Purpose

    A zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette. Contains red eye marker, and is flanked with I-SceI sites.

    Depositor

    Nikolay Ninov

    Article

    Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

    Insert

    Brainbow1.0L
    

    Use

    Zebrafish

    Promoter

    insulin

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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