We narrowed to 19,528 results for: MUT

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pcDNA3-HA-PQBP1

Plasmid

#25804

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 (PQBP1 Human)

Tags

6xHA

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
TPC2(E643A)-mCherry

Plasmid

#135187

Purpose

Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Mutation of glutamate 643 to alanine

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shOGDH 2497

Plasmid

#110942

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

Lentiviral

Promoter

H1/TO

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-GTF2IRD1__ALK

Plasmid

#116821

Purpose

Lentiviral expression of GTF2IRD1__ALK fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GTF2IRD1__ALK

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBK086

Plasmid

#183145

Purpose

pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shOGDH 1714

Plasmid

#110941

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

RNAi

Promoter

H1/TO

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pC-BAR-YR

Plasmid

#61766

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Bar gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBK092

Plasmid

#183146

Purpose

pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS

Plasmid

#164565

Purpose

SARS-CoV-2 Spike protein (S-GSAS variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-GSAS variant)

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRS0045

Plasmid

#131124

Purpose

Mammalian Target-ACE expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1, TadA, and UGI

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-GRM3-VN

Plasmid

#98965

Purpose

FLAG-tagged human mGluR3 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

GRM3 (GRM3 Human)

Tags

FLAG (dykddddk) epitope tag, Signal/leader sequen…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shOGDH 2956

Plasmid

#110943

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

Lentiviral and RNAi

Promoter

H1/TO

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Article

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_linker_mKate2

Plasmid

#167335

Purpose

Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST17-cDAXX

Plasmid

#169729

Purpose

Bacterial expression of N-terminally 6His tagged cDAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

6 His

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-L26A,F27A,W433A,L436A,F437A

Plasmid

#89085

Purpose

mammalian expression of human androgen receptor with mutations of N-terminal FxxLF and WxxLF motifs

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

hAR FxxLF WxxLF mutant (AR Human)

Expression

Mammalian

Mutation

hAR-L26A,F27A,W433A,L436A,F437A

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
Strep-His-Tev-hCGN

Plasmid

#236512

Purpose

For expression of Cingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Human)

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCOTS-pyl-GFP(35TAG)

Plasmid

#92047

Purpose

This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

Depositor

Lital Alfonta

Article

Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

Inserts

EGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)

Use

Replicative expression plasmid for cyanobacteria …

Tags

Histag

Mutation

Tyrosine in position 35 of the GFP was mutated f…

Promoter

LeuP (native S. elongatus promoter), PpsbII, and …

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_mKate2

Plasmid

#167334

Purpose

Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) and HMGA …

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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