Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,447 results for: grn

Clear keywords
Showing: 7561 - 7580 of 15447 results

  2. pADH100

    Plasmid
    #90980
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    90980-plasmid-map-sequence-id-180507

    Enlarge

  4. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

    Enlarge

  5. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Publication
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    164559-plasmid-map-sequence-id-330205

    Enlarge

  12. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

    Enlarge

  14. pX335-NEAT1_IS_v2

    Plasmid
    #97086
    Purpose
    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.
    Depositor
    Archa Fox
    Publication
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA NEAT1_IS_v2
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97086-plasmid-map-sequence-id-178717

    Enlarge

  15. pX335-NEAT1_IS_v1

    Plasmid
    #97085
    Purpose
    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.
    Depositor
    Archa Fox
    Publication
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA NEAT1_IS_v1
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97085-plasmid-map-sequence-id-189638

    Enlarge

  16. pX330-NEAT1m_v1

    Plasmid
    #97083
    Purpose
    Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform
    Depositor
    Archa Fox
    Publication
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA NEAT1m_v1
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    97083-plasmid-map-sequence-id-189494

    Enlarge

  17. Oct4-Cr1

    Plasmid
    #66989
    Purpose
    Expresses gRNA that targets stop codon for OCT4
    Depositor
    Danwei Huangfu
    Publication
    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA for Crispr targeting the OCT4 stop codon
    Expression
    Mammalian
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86232-plasmid-map-sequence-id-177242

    Enlarge

  20. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

    Enlarge

Showing: 7561 - 7580 of 15447 results