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Showing: 1851 - 1900 of 6776 results

  1. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid
    #185918
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185918-plasmid-map-sequence-id-367359

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  2. pB6-Id1-T2A-sfGFP-iCre-ERT2-FNF-TK

    Plasmid
    #139279
    Purpose
    B6J Id1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    C-terminal fusion of T2A-sfGFP-iCre-ERT2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    139279-plasmid-map-sequence-id-315358

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  12. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121799
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121799-plasmid-map-sequence-id-237303

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  13. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid
    #121803
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + EF1a::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121803-plasmid-map-sequence-id-237312

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  14. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid
    #121807
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    polyA + RIP::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121807-plasmid-map-sequence-id-237316

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  25. p09008_AF1-4+GFP5-7

    Plasmid
    #173715
    Purpose
    Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.
    Depositor
    Johannes Kabisch
    Article
    Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8
    Inserts
    hybrid GFP
    mKate2
    Use
    E. coli cell free protein synthesis extracts
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    173715-plasmid-map-sequence-id-361303

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  28. pQdCas12a.luxR(mut)-sggfp(C)

    Plasmid
    #236043
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236043-plasmid-map-sequence-id-471082

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  29. pQdCas12a.luxR(mut)-sggfp(B)

    Plasmid
    #236041
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236041-plasmid-map-sequence-id-469952

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  37. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

    Plasmid
    #189630
    Purpose
    Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.
    Depositor
    Jerzy Szablowski
    Article
    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Inserts
    Gaussia luciferase fused to Fc
    IRES-EGFP
    Use
    AAV, Cre/Lox, and Luciferase
    Tags
    Gluc and IgG1 Fc
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and hSyn
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    189630-plasmid-map-sequence-id-376528

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  39. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154770
    Purpose
    plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, G1 PylT with A41AA and C5…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154770-plasmid-map-sequence-id-309867

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  44. AAVS1-TRE-GFP-TurboID-HA-rtTA

    Plasmid
    #226996
    Purpose
    Integrative plasmid to express GFP-TurboID in mammalian cells
    Depositor
    Bruno Dallagiovanna
    Insert
    Green fluorescent protein (gfp Synthetic)
    Use
    Tags
    TurboID-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    226996-plasmid-map-sequence-id-451925

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  49. f-CTS-EGFP

    Plasmid
    #86064
    Purpose
    A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains ciliary targeting signal (3870-3887 amin…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86064-plasmid-map-sequence-id-179754

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Showing: 1851 - 1900 of 6776 results