We narrowed to 14,552 results for: eng
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Plasmid#167928PurposeDoxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.DepositorInsertT2A-EGFP
UseCRISPR and LentiviralExpressionMammalianPromotertTRE, hPGKAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP
Plasmid#172221PurposeCas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.DepositorTypeEmpty backboneUseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMarsL274G
Plasmid#63177PurposeThis plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMarsL274G (Mars1 Mouse)
ExpressionMammalianMutationL274GPromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mKate2
Plasmid#133452PurposeExpresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mKate2
TagsGSGSGS linker and His6ExpressionBacterialPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP
Plasmid#32481PurposeCre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertPSAML141F,Y115F-GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX459V2.0-HypaCas9
Plasmid#108294PurposepX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
ExpressionBacterialAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLH-sgRNA1-2XPP7
Plasmid#75390PurposesgRNA1-2XPP7DepositorInsertsgRNA1-2XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DRH335: FCK-Optopatch1
Plasmid#51695PurposeCoexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiologyDepositorInsertOptopatch1
UseLentiviralExpressionMammalianPromoterCaMKIIaAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CMV-Nrf2
Plasmid#67636PurposeAAV vector expressing Nrf2 geneDepositorAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET42b-BE3
Plasmid#87437PurposeExpresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMaRSC
Plasmid#89189PurposeThe pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFlag-L274G-T2A-mCherry (Mars1 Mouse)
Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBMN(CMV-copGFP-Luc2-Puro)
Plasmid#80389PurposeTo make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection markerDepositorInsertcopGFP-T2A-Luc2-F2A-Puromycin
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCherry-FRB
Plasmid#25920DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRB (MTOR Human)
TagsmCherryExpressionMammalianAvailable sinceSept. 10, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM200
Plasmid#13079DepositorInsertPtac-gam-bet-exo operon
ExpressionBacterialAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET42b-HF-BE3
Plasmid#87438PurposeExpresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSI-Target-AID-NG
Plasmid#119861PurposeExpresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
ExpressionMammalianAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP
Plasmid#111581PurposeAn AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyrTagRFP-T-P2A-post-eGRASP
UseAAVPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by