We narrowed to 6,508 results for: nls

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pCL33-decoder

Plasmid

#159451

Purpose

Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1mut-tetR-nls-malE-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1mut

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-Jmjd3-F

Plasmid

#17440

Purpose

Expression of Jmjd3 in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

JMJD3 (Kdm6b Mouse)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pRD190

Plasmid

#163100

Purpose

Expression of mEos3.2 in eukaryotes to stain the nuclear compartment

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mEos3.2_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L1-dMb2Cas12a

Plasmid

#177683

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TO-ASCL1-DLX2

Plasmid

#182307

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

van Voorst et al bioRxiv [Preprint]. 2025 Jul 7:2025.06.3

Inserts

Ascl1 (Ascl1 Mouse)
Dlx2 (Dlx2 Mouse)
puroR-T2A-mycNLS-mTagBFP2

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and pTRE3G-bidirectional

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pB-dCas9-PBE

Plasmid

#98159

Purpose

Expresses CRISPR base editor in maize

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Use

Maize

Expression

Plant

Promoter

Ubi

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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L1_ARF19_2

Plasmid

#195914

Purpose

Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19-PhiC31-NLS-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P2

Plasmid

#195918

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3-PhiC31-NLS-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P17

Plasmid

#195922

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PhiC31-NLS-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P22

Plasmid

#195925

Purpose

Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S-PhiC31-NLS-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P78

Plasmid

#195939

Purpose

Level 1 pARF19 reporter : pARF19-mScarlet-NLS-tRBCS

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19-mScarlet-NLS-tRBCS

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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eIscB-D

Plasmid

#227017

Purpose

Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with HMD-D domain and extra SV40 NLS driven by CMV promoter

Depositor

Dali Li

Article

Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

Insert

SV40 NLS-HMG-D-linker-OgeuIscB-v3-SV40-nucleoplasmin NLS

Mutation

D96R/E84R/V159R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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yWL229 MCP-GFP-eRF3

Plasmid

#197053

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-eRF3

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-eRF3

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBK-EE-BE3

Plasmid

#85175

Purpose

Expresses EE-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

EE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5_NLS-Halo-eGFP-BASU

Plasmid

#218449

Purpose

expressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BASU

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPD684 VPR-FRB (no NLS)

Plasmid

#138851

Purpose

Constitutive expression of VPR-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD683 VP64-FRB (no NLS)

Plasmid

#138850

Purpose

Constitutive expression of VP64-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPD682 VP16-FRB (no NLS)

Plasmid

#138849

Purpose

Constitutive expression of VP16-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

Plasmid

#168216

Purpose

Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

SV40 NLS SpCas9

Use

Lentiviral

Tags

HA, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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