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Showing: 741 - 760 of 53924 results

  1. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid
    #138525
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    Control non-PRC2 binding A-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138525-plasmid-map-sequence-id-292292

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  3. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid
    #138524
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    PRC2-binding G-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138524-plasmid-map-sequence-id-292291

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  9. Human Coxsackievirus A16 strain G10 2A protease

    Plasmid
    #228632
    Purpose
    Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Enterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228632-plasmid-map-sequence-id-461401

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  13. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Human, Bovine, Mouse)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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Showing: 741 - 760 of 53924 results