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pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_A1111

Plasmid

#91032

Purpose

Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Promoter

OsAct1

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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cr3Dd

Plasmid

#184862

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, bet, exo

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Ub(L40)

Plasmid

#52892

Purpose

Aedes aegypti ubiquitin promoter driving firefly luciferase

Depositor

Zach Adelman

Article

Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

Insert

ubiquitin promoter

Use

Luciferase

Promoter

Ae aegypti ubiquitin

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pPRIME-dsRed-shscramble

Plasmid

#71384

Purpose

Expresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cells

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

CMV promoter-dsRed-mir30-shscramble

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV enhancer/promoter

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

MBP promoter fragment (Mbp Mouse)

Expression

Mammalian

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pZa pBAD csgA CBDcex pLac Ara csgGEF

Plasmid

#226378

Purpose

Plasmid carries csgA and csgG,E,F genes of curli operon

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

csgA and csgG,E,F genes of curli operon

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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RCAN1

Plasmid

#65413

Purpose

Full length RCAN1 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1 (Rcan1 Mouse)

Tags

Flag tagged

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

Plasmid

#49772

Purpose

Level 2 construct

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

sgRNA_PDS2-Cas9-sgRNA_PDS1

Use

CRISPR

Expression

Plant

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-GFP-Tdtomato-iRFP670

Plasmid

#111812

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
Tdtomato
iRFP670

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLVTH-sip53

Plasmid

#12239

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

shRNA against p53 (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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3279 pSFFV-neo Bid human

Plasmid

#8766

Depositor

Stanley Korsmeyer

Article

Wang et al Genes Dev 1996 Nov 15;10(22):2859-69.

Insert

Bid ORF cDNA FL (BID Human)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
HA-Mob

Plasmid

#32835

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mob (MOB1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242/287A

Plasmid

#32822

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242/287A

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pTK-RetE1-Citrine

Plasmid

#127779

Purpose

Enhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

RetE1

Use

Enhancer reporter construct

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2369 - intron GG1 - 250bp

Plasmid

#159877

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG1 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPM1003

Plasmid

#167550

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Ogawa serotype

Use

Cosmid

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-Pak3(60-113)/S74A/F84A-mCherry-C1

Plasmid

#29676

Depositor

Ryohei Yasuda

Article

Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

Insert

Pak3 (PAK3 Human)

Tags

mCherry

Expression

Mammalian

Mutation

aa60-113 only; S74A F84A

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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Donor_eGP2AP_RC

Plasmid

#133784

Purpose

Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

Depositor

Sriram Kosuri

Article

Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

Type

Empty backbone

Tags

eGFP-P2A-Puro

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-VHH[GFP]

Plasmid

#163856

Purpose

Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

VHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-CD44b-TurboID

Plasmid

#163850

Purpose

In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

CD44b (cd44b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-SERT-myc

Plasmid

#107456

Purpose

Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Serotonin Transporter (SERT) (SLC6A4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag YAP S381A

Plasmid

#27377

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

S381A

Available Since

March 4, 2011

Availability

Academic Institutions and Nonprofits only
pKanCMV-mClover3-10aa-H2B

Plasmid

#74257

Purpose

Mammalian expression of N-terminal fusion of mClover3 to H2B

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

H2B

Tags

mClover3

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Ataxin3Q84

Plasmid

#22124

Depositor

Henry Paulson

Article

Chai et al J Neurosci. 1999 Dec 1. 19(23):10338-47.

Insert

Ataxin 3 (ATXN3 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
tol2-mpx-lyn-GFP

Plasmid

#35037

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

Lyn-GFP (lyn Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

Plasmid

#69906

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (CDR1-AS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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