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Showing: 7581 - 7600 of 65059 results

  3. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84261
    Purpose
    Expresses AND gate ABA/GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9
    PYL1-VPR-p2a-GID1-ABI
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84261-plasmid-map-sequence-id-160892

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  4. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

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  5. pICE-HA-MRE11-H129N

    Plasmid
    #82034
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Nuclease-dead mutant: H129N MRE11
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82034-plasmid-map-sequence-id-155387

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  14. pTPGI_dCas9

    Plasmid
    #64279
    Purpose
    encodes yeast-optimized dCas9 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    Yeast-optimized dCas9
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    D10A & H840A relative to S. pyogenes Cas9
    Promoter
    pTPGI
    Available Since
    May 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    64279-plasmid-map-sequence-id-107786

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  17. pMV306DIG13+FFlucRT

    Plasmid
    #49998
    Purpose
    Integrase removed to increase stability of the plasmid in mycobacteria
    Depositor
    Brian Robertson
    Article
    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2
    Insert
    G13 promoter + Firefly luciferase Red Thermostable
    Use
    Mycobacteria integrating vector
    Expression
    Bacterial
    Mutation
    Codon optimized for Mycobacterium tuberculosis wi…
    Promoter
    G13
    Available Since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    49998-plasmid-map-sequence-id-74812

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  19. pcDNA3.1 Flag YFP hNKCC1 (NT17)

    Plasmid
    #49085
    Purpose
    Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available Since
    Nov. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    49085-plasmid-map-sequence-id-72211

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Showing: 7581 - 7600 of 65059 results