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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,359 results for: nts

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Showing: 7661 - 7680 of 15359 results

  1. pcDNA4/TO-Flag-H3.3

    Plasmid
    #47980
    Purpose
    tetracycline-inducible expression of human Flag tagged H3F3A
    Depositor
    Bing Zhu
    Article
    Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:
    Insert
    H3F3A (H3-3A Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    288 bp T to C synonymous mutation
    Promoter
    CMV promoter with tetracycline operator
    Available Since
    Sept. 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available Since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62204-plasmid-map-sequence-id-101817

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  3. Adeno EA

    Plasmid
    #64071
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Insert
    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Promoter
    U6 and CBh
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91130-plasmid-map-sequence-id-182254

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  18. pCMV-MtAcKRS (human-opti)

    Plasmid
    #164195
    Purpose
    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion
    Depositor
    Tao Liu
    Article
    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2
    Insert
    MtAcKRS-478(human-opti)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    D76G; L325M; L329I; Y330F; L333A; C372F
    Promoter
    CMV
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164195-plasmid-map-sequence-id-325763

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  19. pAW212

    Plasmid
    #172196
    Purpose
    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites
    Depositor
    Chang Liu
    Article
    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038
    Insert
    p10B2-AT110
    Expression
    Yeast
    Promoter
    10B2 (P1 specific promoter)
    Available Since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172196-plasmid-map-sequence-id-338565

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  20. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

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Showing: 7661 - 7680 of 15359 results