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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-dLight1.3b

Plasmid

#135762

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

Synapsin

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-3XFLAG-miniTurbo-NLS-VHL54:213

Plasmid

#248671

Purpose

To generate inducible Flp-In cells expressing Nterm tagged miniTurbo deltaVHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL(54-213) (VHL Human)

Tags

FLAG-miniTurbo-NLS

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-NLS-VHL-miniTurbo-3xFLAG

Plasmid

#248670

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo full length VHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL (VHL Human)

Tags

FLAG-miniTurbo and NLS

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100851

Purpose

AAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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VAMP7 HA-delta pMEP4

Plasmid

#62958

Purpose

full length human VAMP7 with C-terminal HA tag cloned into delta pMEP4

Depositor

Paul Luzio

Article

Pryor et al Cell. 2008 Sep 5;134(5):817-27. doi: 10.

Insert

VAMP7 (VAMP7 Human)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pLL5.0-MRLC-DD-GFP

Plasmid

#187260

Purpose

Lentiviral expression of myosin regulatory light chain-DD mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Mutation

T18D, S19D

Promoter

5' LTR

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBac1.tR4-AcdRS82

Plasmid

#174101

Purpose

Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

M. barkeri tRNA synthetase for acridone82

Expression

Mammalian

Mutation

N311S, C313G, V366A, W382T

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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WDR5-HaloTag

Plasmid

#200881

Purpose

Mammalian expression of human WDR5 with C-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

WDR5-HaloTag (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.1

Plasmid

#111067

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.1

Use

AAV

Tags

Flag tag

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only
CAG FLEX BARK D110A p2A mCherry

Plasmid

#117694

Purpose

CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

Cre/Lox

Tags

HA / FLAG

Mutation

D110A

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO-2TO

Plasmid

#86697

Purpose

Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 2TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Plasmid

#35507

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-GST-4EBP1-Low GC

Plasmid

#69018

Purpose

expresses tagged 4EBP1

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

EIF4EBP1 (EIF4EBP1 Human)

Tags

GST and HA

Expression

Mammalian

Mutation

lowGC content, wt is ~70% GC rich. Still, it code…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC-FLAG

Plasmid

#129494

Purpose

Expresses human TMEM206 tagged at the C-terminus with FLAG

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER13 (miR-LUP)

Plasmid

#46936

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

Plasmid

#35510

Purpose

AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

Plasmid

#35500

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

Plasmid

#35512

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV2

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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GST-Rab8a-QL

Plasmid

#86079

Purpose

A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Mutation

Changed glutamine 67 to leucine

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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