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Nkx6.2

Plasmid

#15542

Depositor

John Rubenstein

Article

Qiu et al Mech Dev. 1998 Mar . 72(1-2):77-88.

Insert

Nkx6.2 (Nkx6-2 Mouse)

Expression

Bacterial

Mutation

Partial cDNA

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Myc-His(-)-MSulf-1

Plasmid

#13007

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

mouse sulfatase 1 (Sulf1 Mouse)

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
RCAS DNhRARalpha (CC#1865)

Plasmid

#15153

Depositor

Connie Cepko

Article

Sen et al Development. 2005 Dec . 132(23):5147-59.

Insert

RAR alpha (dom neg) (RARA Human)

Use

Retroviral; Avian expression

Mutation

Dominant negative. Truncated at aa403, so lacks t…

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pMax_mPlum

Plasmid

#177830

Purpose

Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV5)

Viral Prep

#198516-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Promoter

EF-1a

Tags

mScarlet

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only
IVT-donor_opt(R2Tg)-GFP

Plasmid

#223246

Purpose

In vitro transcription of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

In vitro transcription

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_G299C

Plasmid

#177829

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_G299C

Expression

Mammalian

Mutation

G299C

Promoter

CMV

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-mCherry

Plasmid

#49647

Purpose

TALERed-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

mCherry

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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TFORF3412

Plasmid

#144888

Purpose

Lentiviral vector for overexpressing the EGR1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EGR1 (EGR1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-SURF4

Plasmid

#170680

Purpose

Expresses FLAG-tagged SURF4 in mammalian cells.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

SURF4

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_Ubiquitin

Plasmid

#131258

Purpose

Mammalian expression of ubiquitin with an N-terminal HA tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xFLAG-HSP27

Plasmid

#129229

Purpose

Encodes FLAG tagged human HSP27 for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

HSP27 (HSPB1 Human)

Tags

FLAG and GST

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1

Plasmid

#108127

Purpose

Recruitable SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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Stx4-pmCitrine

Plasmid

#92422

Purpose

Expression of syntaxin-4 C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx4 (Stx4 Rat)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pECE M2-SNX17 WT

Plasmid

#69811

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn1-AP-His

Plasmid

#71978

Purpose

Expresses the entire Netrin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn1 (Ntn1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)

Plasmid

#72273

Purpose

FLAG-Mnase expressed under the REB1 promoter

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Promoter

REB1

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pCDH_SBP-EGFP-Ecadherin

Plasmid

#65290

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-CTR1

Plasmid

#53157

Purpose

Expresses human CTR1 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only