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Puro.let-7g.Cre

Plasmid

#17409

Purpose

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Let-7g

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-ConFonvConNon-eGFP-W3SL

Plasmid

#218753

Purpose

Provides AND intersectional (Cre+Flp+vCre+Vika) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-RGECO1.0

Plasmid

#175813

Purpose

The lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

RGECO1.0

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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5HT2A-APEX2

Plasmid

#215558

Purpose

Stable, inducible expression of 5HT2A

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

35S

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSL1-E2C

Plasmid

#180421

Purpose

Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.

Depositor

Nancy Moran

Article

Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

E2 Crimson

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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PEP109E

Plasmid

#97395

Purpose

binary construct of cytosolic mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

cytosol

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1

Plasmid

#221494

Purpose

NKX2-1 short isoform lentiviral overexpression using hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCD/NL-BH*deltavpu/RT-

Plasmid

#136985

Purpose

Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

Depositor

Jakob Reiser

Article

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif

Expression

Mammalian

Mutation

carries D110E mutation in the reverse transcripta…

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHK352.3

Plasmid

#235473

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

Plasmid

#225924

Purpose

Expressing LGC-47 in AVA neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

rig-3

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM60-Cnbp

Plasmid

#38246

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Cnbp (Cnbp Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
OA-1067B

Plasmid

#200252

Purpose

pBac-U6-gRNA(doublesex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

4 gRNAs targeting Doublesex

Expression

Insect

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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PfICH pET15b

Plasmid

#60684

Purpose

Recombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescens

Depositor

Mark Wilson

Article

Lakshminarasimhan et al J Biol Chem. 2010 Sep 17;285(38):29651-6

Insert

inhA

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pIS1-Vim5UTR-renilla

Plasmid

#38237

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim 5'UTR / Renilla (Vim Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Vim 5' UTR fused to renilla ORF

Promoter

Endogenous Vim

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP GFP

Plasmid

#45238

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, GFP

Tags

GFP and full length CENP-B

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-PKG-hNo.7

Plasmid

#175812

Purpose

The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

PKG #7

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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