We narrowed to 10,212 results for: FIE
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Plasmid#172504PurposeEncodes the GwCas7x3 protein only for bacterial recombinant protein expression for purificationDepositorInsertGwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVenus-Sec16A
Plasmid#36157PurposeExpresses Sec16A as a Venus FP fusionDepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-ER-EmEGFP
Plasmid#169000PurposeExpress ER-EmEGFP in zebrafishDepositorInsertER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)
UseUnspecifiedTagsEGFPAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mRFP1 gRNA
Plasmid#117052PurposeIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoterDepositorInsertanti-mRFP1 SpCas9 gRNA
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125236PurposeExpresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cellsDepositorInsertβ2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-CSK-R107E
Plasmid#74503PurposeOverexpression of CSK R107E mutant in mammalian cellsDepositorAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'Q-mito
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB321
Plasmid#68649PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-Nvec-codonopti-Cas9-2xNLS
Plasmid#141108PurposePlasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensisDepositorInsertNematostella codon optimized Cas9
UseUnspecifiedMutationAll amino acids coded for by codons optimized for…PromoterT7Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD102
Plasmid#131403PurposeE. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD102
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
CKAP2-mNeonGreen pHAT
Plasmid#175947PurposeBacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
E1010m (RFP)_CD
Plasmid#66033PurposeMoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]DepositorInsertFluorescent reporter - RFP
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB121
Plasmid#68575PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
DTECT - Plasmid for Standard Curve
Plasmid#139333PurposeThis plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECTDepositorInsertDTECT ligation product
UseUnspecifiedAvailable SinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE2-Sox10-FLAG-tev-AVI
Plasmid#127775PurposeModified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tagDepositorAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0761
Plasmid#185630PurposeModified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-CMV-Cdc42 3'UTR
Plasmid#51389DepositorAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Mus.1
Plasmid#196681PurposeRep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationWVLPSGG insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MDV1B
Plasmid#196680PurposeRep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationKTVGTVY insert between amino acids 588 and 589 of…Promoterp41Available SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only