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pUdO2te

Plasmid

#210243

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOanc-a

Plasmid

#210320

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-RGD-STReTCh (Force Inert)

Plasmid

#216710

Purpose

Expresses HaloTag-RGD-STReTCh Force Inert Control in bacteria

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

HaloTag-RGD-STReTCh

Tags

6xHis and HaloTag

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag KO sgRNA

Plasmid

#207102

Purpose

pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTCGATGTTGGTCCGCGCGA

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #2

Plasmid

#207106

Purpose

pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CTGAAGGAACAGACTAATTC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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53BP1 KO sgRNA

Plasmid

#207104

Purpose

pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGATTCTCAGCCTGAAAGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-LASV-GPC-HA-c3xFLAG

Plasmid

#215691

Purpose

Produces the Lassa virus glycoprotein with a c-terminal HA tag and 3xFLAG tag

Depositor

Melinda Brindley

Article

Willard et al Pathogens. 2018 Dec 26;8(1):1. doi: 10.3

Insert

LASV GP-HA-FLAG

Tags

HA-3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUCmini-NFKB1-PGK-EGFP

Plasmid

#204018

Purpose

Homologous donor template for NFKB1 knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB1-PGK-tdTomato

Plasmid

#204019

Purpose

Homologous donor template for NFKB1 knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB2-PGK-EGFP

Plasmid

#204020

Purpose

Homologous donor template for NFKB2 knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB2-PGK-tdTomato

Plasmid

#204021

Purpose

Homologous donor template for NFKB2 knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211863

Purpose

Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA

Mutation

C492A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO2t

Plasmid

#210247

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO1t

Plasmid

#210246

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO2s

Plasmid

#210239

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO2k

Plasmid

#210235

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO4te

Plasmid

#210245

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdO1te

Plasmid

#210242

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge