We narrowed to 18,304 results for: pho;

1. 6xHis-SNAP-PI(3,4,5)P3 Probe

   Plasmid

   #211514

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BTK1-PH
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG/ATP10D(E215Q)-HA

   Plasmid

   #209248

   Purpose

   Mammalian expression of ATP10D mutant E215Q

   Depositor

   Hye-Won Shin

   Article

   Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

   Insert

   ATP10D (ATP10D Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E215Q

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

   Plasmid

   #114595

   Purpose

   Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

   Depositor

   Brian Chow

   Article

   Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

   Insert

   BcLOV4
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-Cdk9

   Plasmid

   #28100

   Depositor

   Andrew Rice

   Article

   Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-PH-FAPP1

   Plasmid

   #161986

   Purpose

   Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

   Depositor

   David Bryant, Tamas Balla

   Article

   Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

   Insert

   PH-FAPP1 (PLEKHA3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-PIPK1 gamma 90

   Plasmid

   #22299

   Depositor

   Pietro De Camilli

   Article

   Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

   Insert

   human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-FOXO3a TM

   Plasmid

   #1788

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   HA (YDVPDYASL)

   Expression

   Mammalian

   Mutation

   T32A, S253A, S315A (no longer detectably phosphor…

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-PIPK1 gamma 87

   Plasmid

   #22300

   Depositor

   Pietro De Camilli

   Article

   Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

   Insert

   human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Grp1-PH pGEX2TK

   Plasmid

   #71379

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 264-391

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-HA-ERK5

    Plasmid

    #65244

    Purpose

    Expression of HA tagged ERK5 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

    Insert

    ERK5 (MAPK7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A409T

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHJ423

    Plasmid

    #20681

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Thr 567 to Asp

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 PARL-FLAG-CT S277G

    Plasmid

    #13615

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed catalytic Serine 277 to Alanine

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid

    #181833

    Purpose

    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dPlcR (K30L,K32L)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSecTag-ATX

    Plasmid

    #17839

    Depositor

    Steven Rosen

    Article

    Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

    Insert

    Autotaxin (ENPP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

15. GFP-CRY2-OCRL

    Plasmid

    #79565

    Purpose

    expression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL

    Depositor

    Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    OCRL (OCRL Human)
    

    Use

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    aa 234_539

    Promoter

    CMV

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-OSH2-2xPH

    Plasmid

    #161987

    Purpose

    Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

    Depositor

    David Bryant, Tamas Balla

    Article

    Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

    Insert

    OSH2 (OSH2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-Lyn-dInPAkt(R23/25A)

    Plasmid

    #181835

    Purpose

    Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dInPAkt
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Arginines 23 and 25 in PH domain mutated to alani…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGex-Cav1

    Plasmid

    #33364

    Depositor

    Ambra Pozzi

    Article

    Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

    Insert

    Cav1 (CAV1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pIA1362

    Plasmid

    #166860

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp7 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSecTag-T210A-ATX

    Plasmid

    #17840

    Depositor

    Steven Rosen

    Article

    Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

    Insert

    T210A-ATX (ENPP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pBS hBAF53a

    Plasmid

    #17879

    Depositor

    Jerry Crabtree

    Article

    Zhao et al Cell. 1998 Nov 25. 95(5):625-36.

    Insert

    BAF53a (ACTL6A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3-HA-ERK5kin

    Plasmid

    #65245

    Purpose

    Expression of HA tagged ERK5 kinase domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

    Insert

    ERK5kin (MAPK7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 410-817

    Promoter

    CMV

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid

    #22931

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaC (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PHLPP-deltaC, deletion of PDZ binding domain--the…

    Promoter

    CMV

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3-Lyn-PlcR(K30/32L)

    Plasmid

    #181831

    Purpose

    Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-PlcR (K30L,K32L)
    

    Use

    Tags

    ECFP, EYFP, and N-terminal targeting sequence fro…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HA-Cdk9 T186A

    Plasmid

    #28103

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-BD-cop1at(335-675)

    Plasmid

    #44973

    Depositor

    Thanos Halazonetis

    Article

    Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

    Insert

    BD-COP1 (COP1 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-334

    Promoter

    pCMV

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHJ424

    Plasmid

    #20682

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pGEX4T2-Snf1-cat

    Plasmid

    #52683

    Purpose

    expression of the Snf1 kinase domain in bacteria

    Depositor

    Benjamin Turk

    Article

    Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

    Insert

    SNF1 (SNF1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes kinase domain only, aa 41-315

    Promoter

    tac

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pGEX2TK-YAP-WW1-RRK

    Plasmid

    #104306

    Purpose

    bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated

    Depositor

    Marius Sudol

    Insert

    YAP WW1 domain (YAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…

    Promoter

    tac

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge