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Showing: 1951 - 2000 of 49419 results

  4. enIscB

    Plasmid
    #205410
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    205410-plasmid-map-sequence-id-421947

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  13. pYES2-gRNA-hyg-MCS

    Plasmid
    #107734
    Purpose
    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.
    Depositor
    Sheng Yang
    Article
    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.
    Insert
    X-3 site gRNA expression cassettes
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    107734-plasmid-map-sequence-id-208539

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  17. pP[RS3]3'M

    Plasmid
    #53552
    Purpose
    for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila
    Depositor
    Renjie Jiao
    Article
    Yu et al Biol Open. 2014 Apr 15;3(4):271-80. doi:
    Insert
    AscI and MluI restriction enzyme sites
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    same pP[RS3]
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. TadA-8e-eIscBn

    Plasmid
    #227018
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227018-plasmid-map-sequence-id-453983

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  19. IscB-v3

    Plasmid
    #227015
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    SV40 NLS-OgeuIscB-v3-nucleoplasmin NLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227015-plasmid-map-sequence-id-453982

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  20. eIscB

    Plasmid
    #227016
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with extra SV40 NLS driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    SV40 NLS-OgeuIscB-v3-SV40-nucleoplasmin NLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227016-plasmid-map-sequence-id-453979

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  22. pGES201

    Plasmid
    #190197
    Purpose
    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection
    Depositor
    Yuefeng Guan
    Article
    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190197-plasmid-map-sequence-id-371938

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  23. enIscB-T5E

    Plasmid
    #205411
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205411-plasmid-map-sequence-id-416557

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  24. miCBE

    Plasmid
    #205413
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205413-plasmid-map-sequence-id-416749

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  28. PET28-FnCas12a-EP16

    Plasmid
    #183636
    Purpose
    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
    Depositor
    Yunzi Luo
    Article
    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-
    Insert
    FnCas12a-EP16
    Use
    CRISPR
    Tags
    6Ă—His Tag
    Expression
    Bacterial
    Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
    Promoter
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183636-plasmid-map-sequence-id-375575

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  32. eiABE

    Plasmid
    #227144
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227144-plasmid-map-sequence-id-453987

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  33. TadA-8e-eIscBn-C-HMG-D

    Plasmid
    #227145
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-linker-HMG-D-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227145-plasmid-map-sequence-id-453986

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  34. eIscB-D

    Plasmid
    #227017
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with HMD-D domain and extra SV40 NLS driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    SV40 NLS-HMG-D-linker-OgeuIscB-v3-SV40-nucleoplasmin NLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227017-plasmid-map-sequence-id-453985

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  35. hA3A*-IscBn

    Plasmid
    #227146
    Purpose
    Vector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227146-plasmid-map-sequence-id-453980

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  36. eiCBE

    Plasmid
    #227148
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227148-plasmid-map-sequence-id-453981

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  37. TadA-8e-eIscBn-N-HMG-D

    Plasmid
    #227143
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227143-plasmid-map-sequence-id-453972

    Enlarge

  45. hA3A*-eIscBn-D

    Plasmid
    #227147
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant and HMG-D domain was inserted between hA3A* and eIscBn driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-HMG-D-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227147-plasmid-map-sequence-id-453978

    Enlarge

  48. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205412-plasmid-map-sequence-id-416556

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Showing: 1951 - 2000 of 49419 results