We narrowed to 1,380 results for: ccdb gene

1. pAG415Gal_Gcn4 R3W

   Plasmid

   #232975

   Purpose

   Galactose iduced expression of Gcn4 R++ W+ in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 R++ W+
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D103R, N126W, D133R, K143R

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 WT 44mer

   Plasmid

   #232956

   Purpose

   Galactose iduced expression of Gcn4 WT 44mer in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WT 44mer
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG415Gal_Gcn4 E3HA

   Plasmid

   #232996

   Purpose

   Galactose iduced expression of Gcn4 E+HA in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 E+
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D105E, D118E, D139E

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG415Gal_Gcn4 WW+

   Plasmid

   #232984

   Purpose

   Galactose iduced expression of Gcn4 WW+ in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WW+
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   E109N, S117D, T121W, N126W, T132W

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG415Gal_Gcn4 F108A+

   Plasmid

   #232974

   Purpose

   Galactose iduced expression of Gcn4 F108A in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 F108A
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   F108A, T121L, A141P

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG415Gal_Gcn4 ILVtoAS

   Plasmid

   #232959

   Purpose

   Galactose iduced expression of Gcn4 ILVtoAS in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoAS
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   L123S, I128S, V130A, V135A, L137S, I142S

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG415Gal_Gcn4 WT 44merGFP

   Plasmid

   #233000

   Purpose

   Galactose iduced expression of Gcn4 WT 44merGFP in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WT 44mer
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG415Gal_Gcn4 WT 30mer

   Plasmid

   #232986

   Purpose

   Galactose iduced expression of Gcn4 WT 30mer in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WT 30mer
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426-GAL-APP-CTF

   Plasmid

   #181732

   Purpose

   Galactose inducible expression of APP-CTF

   Depositor

   Alejandro Chavez

   Insert

   APP-CTF (APP Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C-terminal fragment (aa 671-770)

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAL3

    Plasmid

    #114673

    Purpose

    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.

    Depositor

    William Studier

    Article

    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6

    Type

    Empty backbone

    Use

    Tags

    optional Ser-Gly-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGE1108

    Plasmid

    #202772

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDrop

    Plasmid

    #184872

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDGE1176

    Plasmid

    #202774

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDD268

    Plasmid

    #132523

    Purpose

    mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mNG-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mNG

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLOVE-GFP

    Plasmid

    #15949

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

    Insert

    Green Fluorescent Protein
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Promoter

    Available Since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pDD283

    Plasmid

    #66824

    Purpose

    YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    YPET-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized YPET

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKEB31

    Plasmid

    #31224

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    ccdB and Cm resistance gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. mito cpVenus control

    Plasmid

    #186792

    Purpose

    Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    mito cpVenus control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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