Skip to main content
Addgene
Showing: 781 - 800 of 8751 results

  3. qTAG-AAVS1-Ef1a-Zeo

    Plasmid
    #227268
    Purpose
    Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227268-plasmid-map-sequence-id-451274

    Enlarge

  11. EF1a-EGFP (no signal control)

    Plasmid
    #170167
    Purpose
    Mammalian Expression of no signal control
    Depositor
    Christopher Moxham
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    170167-plasmid-map-sequence-id-338528

    Enlarge

  15. AAV-EF1a-jGCaMP8f-WPRE

    Plasmid
    #176756
    Purpose
    AAV-mediated expression of GCaMP8f under the EF1a promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176756-plasmid-map-sequence-id-349049

    Enlarge

  17. pAAV-Ef1a-fDIO mCherry

    Plasmid
    #114471
    Purpose
    Flp-dependent mCherry Control
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Ef1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114471-plasmid-map-sequence-id-220928

    Enlarge

  20. pLV-EF1A-CD19.28H.28TM.28.BB.3z

    Plasmid
    #200680
    Purpose
    Modular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD28HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200680-plasmid-map-sequence-id-391913

    Enlarge

Showing: 781 - 800 of 8751 results