We narrowed to 6,574 results for: val

1. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

   Plasmid

   #60910

   Purpose

   dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   dSV40 Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

   Plasmid

   #60904

   Purpose

   The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

   Plasmid

   #60903

   Purpose

   Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   Cas9 dead
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMON-HsNMT1

   Plasmid

   #67475

   Purpose

   Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

   Depositor

   Richard Kahn

   Article

   Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

   Insert

   HsNMT1 (NMT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. GFP-Rab5

   Plasmid

   #31733

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Rab5 (Rab5 Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Actin

   Available since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pTrex-Luc-Neo

   Plasmid

   #48336

   Purpose

   Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

   Depositor

   Rick Tarleton

   Article

   Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

   Insert

   firely luciferase
   

   Use

   Trypanasoma cruzi expression

   Tags

   Expression

   Mutation

   Promoter

   T. cruzi rRNA

   Available since

   July 20, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET17_K560_GFP_His

   Plasmid

   #15219

   Depositor

   Ron Vale

   Article

   Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

   Insert

   Human neuronal kinesin heavy chain (KIF5B Human)
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-Hook3 aa 1-552

   Plasmid

   #74614

   Purpose

   Expresses a truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-552 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-FIP3

   Plasmid

   #74753

   Purpose

   Expressed sfGFP-tagged FIP3 in E. coli for protein production

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab11 family interacting protein 3 (RAB11FIP3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCherry-mNrp1

    Plasmid

    #21934

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Tags

    Cherry and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 human cofilin WT

    Plasmid

    #50853

    Purpose

    expresses human cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pDual-eGFP

    Plasmid

    #63215

    Purpose

    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pHR_dSV40-Aurora A-GFP

    Plasmid

    #67924

    Purpose

    Expresses Aurora A-GFP in mammalian cells

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    GFP-Aurora A (AURKA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-TAT-2-84-FLAG

    Plasmid

    #35980

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV2 84 amino acids

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFasBac+GFP-CAMSAP2

    Plasmid

    #59037

    Purpose

    for baculovirus expression of CAMSAP2 in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 (CAMSAP2 Human)
    

    Use

    Tags

    His and eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFasBac+GFP-CAMSAP1

    Plasmid

    #59036

    Purpose

    for baculovirus expression of CAMSAP1 in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP1 (CAMSAP1 Human)
    

    Use

    Tags

    His and eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GreenT-EC - GPI

    Plasmid

    #193946

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC
    

    Use

    Tags

    GPI-anchor and Igk_secretion - HA-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFasBac+GFP-CAMSAP3

    Plasmid

    #59038

    Purpose

    for baculovirus expression of mouse CAMSAP3 in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 (Camsap3 Mouse)
    

    Use

    Tags

    His and eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EFF-1::V5

    Plasmid

    #132961

    Purpose

    Expresses EFF-1 A full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    EFF-1::V5
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge