We narrowed to 19,275 results for: tec
-
-
SMC2-mAC donor (Hygro)
Plasmid#140648PurposeSMC2 tagging with mAID-CloverDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA
Plasmid#193968PurposeEncodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoterDepositorAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV SNAP MDC1
Plasmid#227375PurposeSNAP–MDC1 expressionDepositorInsertMDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
UseLentiviralTagsSNAPExpressionMammalianAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK-AID-CTERM-GFP
Plasmid#185778PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDMutationnonePromoterPGKAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-DTAG-CTERM-GFP
Plasmid#185765PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGMutationnonePromoterSFFVAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-UL29-egfp-U3-UL8
Plasmid#166694PurposeTo express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).DepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK321 (TOP2A-mAC (Neo))
Plasmid#140652PurposeTOP2A tagging with mAID-CloverDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX
Plasmid#216491PurposeExpression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cellsDepositorInsertHaloAKAR2.1
TagsNES, T2A-EGFP-CAAXExpressionMammalianPromoterCMVAvailable SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE
Plasmid#138143PurposeExpresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caacDepositorInsertPguCas13b
UseLentiviralTagsNLSAvailable SinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE
Plasmid#138146PurposeExpresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caacDepositorInsertPspCas13b
UseLentiviralTagsNESAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCATmut
Plasmid#140085PurposeA control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-HBO1-G485A pc2201
Plasmid#167570PurposeThe human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)DepositorInserthistone acetyltransferase 1 (KAT7 Human)
TagsGFPExpressionMammalianMutationG485APromoterCMVAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
miniABEmax (pRZ900)
Plasmid#131311PurposeCMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).DepositorInsertbpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PET-Sniper SpCas9-NLS-6xHis
Plasmid#207374PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSniper SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationF539S, M763I, K890NPromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)
Plasmid#140533PurposeOsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGK-SMASH-NTERM-GFP
Plasmid#185771PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShMutationnonePromoterPGKAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP2133
Plasmid#72249PurposeHuman expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationY450A, N497A, R661A, Q695A, and Q926APromoterCMVAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-mPlum-HA
Plasmid#200009PurposeDonor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.DepositorInserteGFP, mPlum
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only
