169,450 results

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Human SERCA2a (pcDNA3.1+)

Plasmid

#75187

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, David MacLennan

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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GZnP3 in pcDNA3.1+

Plasmid

#161738

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Article

Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

Insert

GZnP3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Alpha 5 integrin-GFP

Plasmid

#15238

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

alpha 5 integrin (ITGA5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-sNRR-T2A-BFP

Plasmid

#194930

Purpose

LV construct encoding sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-FAK W266A

Plasmid

#186149

Purpose

For expressing FAK in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

Plasmid

#208692

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEvol-MjaYRS

Plasmid

#153557

Purpose

Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

Depositor

Huiwang Ai

Article

Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

Inserts

MJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter

Expression

Bacterial

Promoter

glnS and pBAD

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ152

Plasmid

#69303

Purpose

Gateway entry vector with pco-dCas9-VP64

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-VP64 (Plant codon-optimized)

Use

CRISPR

Mutation

VP64 activator fusion; NLS fusion

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJC005.em

Plasmid

#182738

Purpose

CRISPR-Cas12a genome editing of E. faecium

Depositor

James Collins

Article

Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone

Pooled Library

#1000000075

Purpose

Genome-wide CRISPR gRNA pooled library for activation of mouse genes

Depositor

Feng Zhang

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only
CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_GR-RE_MLPmin_BC1094-luc2

Plasmid

#227114

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_SNAP-tag2

Plasmid

#226512

Purpose

Bacterial cell expression of SNAP-tag2

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

SNAP-tag2

Tags

His-tag (10x) and Strep-Tag II,

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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W118-1_Flag-hIRAK1

Plasmid

#180405

Purpose

lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

IRAK1 (IRAK1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Rich-pT7

Plasmid

#108445

Purpose

This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

Depositor

Richard Bélanger

Type

Empty backbone

Promoter

pT7

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1_SPTLC1_C133W

Plasmid

#181921

Purpose

3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W

Depositor

Christian Metallo

Article

Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

Insert

Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)

Use

Lentiviral; Mammalian expression, , doxycycline i…

Expression

Mammalian

Mutation

cysteine at position 133 of the coding region of …

Promoter

tight TRE promoter

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHaMDRwt

Plasmid

#10957

Depositor

Michael Gottesman

Article

Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

Insert

MDR1 (ABCB1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-hMIGA2-DsRed

Plasmid

#192866

Purpose

Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

DsRed

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SspBmicro-mCh-p60

Plasmid

#190168

Purpose

Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

SspBmicro-mCherry-p60

Tags

SspBmicro-mCherry

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-IRF4

Plasmid

#232667

Purpose

transient expresses IRF4 in mammalian cells

Depositor

Chen-Yang Shen

Insert

IRF4 (IRF4 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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