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pLenti-PGK-RAP1A-E63-puro

Plasmid

#156167

Purpose

Lentiviral vector for expression of RAP1A-E63, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Glutamine 63 to Glutamic acid

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1.sh.beta-catenin.2279

Plasmid

#19762

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against beta-catenin (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
A*68:01:01

Plasmid

#203292

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*68:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A

Plasmid

#166458

Purpose

Expresses fusion of mEGFP and YAPS127A

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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A*02:11:01

Plasmid

#203280

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*02:11:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta EC

Plasmid

#202423

Purpose

Expression of GFP-tagged PODXL without extracellular domain

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

deleted extracellular domain

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL 5N>Q

Plasmid

#202419

Purpose

Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

N33/43/104/144/360Q

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAP

Plasmid

#166443

Purpose

Bacterial expression of 6x His tagged YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Bcl6

Plasmid

#137851

Purpose

Express Bcl6 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

B cell lymphoma 6 (BCL6 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 C-V5-tag ORF3a

Plasmid

#179969

Purpose

Mammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 ORF3a (ORF3a Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pmEos2-N1 GBP (GBP-mEos2)

Plasmid

#162876

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein tagged with mEos2

Tags

mEos2

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 M C-V5-tag

Plasmid

#179978

Purpose

Mammalian expression vector for SARS-CoV-2 M, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 M (M Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFG eIF4G1 +MIC

Plasmid

#158776

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV2 N C-V5-tag

Plasmid

#179979

Purpose

Mammalian expression vector for SARS-CoV-2 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 N (N Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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sgAPC(B)

Plasmid

#85558

Purpose

Expresses sgRNA targeting human APC around codon I1417

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

APC, WNT signaling pathway regulator (APC Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.KD.flag

Plasmid

#19759

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

D173A (kinase dead)

Available Since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only
pLenti-EmGFP-LATS2/1 KD

Plasmid

#52085

Purpose

Lentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporter

Depositor

Yutaka Hata

Article

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

LATS2 and LATS1 miRNAs (LATS1, LATS2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-TEAD4-IDR

Plasmid

#166451

Purpose

Expresses fusion of mCherry and TEAD4-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR(89-230) (TEAD4 Human)

Expression

Mammalian

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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A*66:01:01

Plasmid

#203291

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*66:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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