167,785 results

1. EJ2GFP-puro

   Plasmid

   #44025

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ2GFP egfp-based chromosomal break reporter
   

   Expression

   Mammalian

   Mutation

   insertion of I-SceI, 3 frame stop, and flanking m…

   Promoter

   pCAGGS

   Available Since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-hSynI-rtTAV16

   Plasmid

   #102367

   Purpose

   AAV vector to drive TET-ON activator expression from human synapsin I promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

   Insert

   rtTAV16
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   rtTA variant reported in Gene Therapy (2006) 13, …

   Promoter

   human synapsin I promoter

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_PIK3CA_p.H1047R

   Plasmid

   #82824

   Purpose

   Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Gateway entry vector

   Mutation

   H1047R

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

   Plasmid

   #113691

   Purpose

   U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti puro HA-Ubiquitin

   Plasmid

   #74218

   Purpose

   Lentiviral expression of HA tagged Ubiquitin

   Depositor

   Melina Fan

   Insert

   ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available Since

   March 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

   Plasmid

   #200630

   Purpose

   Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Promoter

   pTRE3G

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #125691

   Purpose

   Global labeling of excitatory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1 beta-catenin WT

   Plasmid

   #16071

   Depositor

   Erin Schuman

   Article

   Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

   Insert

   beta-catenin (CTNNB1 Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   *

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAF0451 Si74_attP-mCherry donor

   Plasmid

   #193470

   Purpose

   Promoterless mcherry donor for Si74

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-FLEX-Volton2-ST-WPRE (AAV1)

    Viral Prep

    #172907-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-Volton2-ST-WPRE (#172907). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-Volton2-ST-WPRE plasmid DNA. Cre-dependent, Syn-driven expression of the voltage sensor Voltron2-ST containing a soma localization targeting sequence. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Promoter

    Syn

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pUB_smHA_KDM5B_MS2

    Plasmid

    #81085

    Purpose

    Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

    Depositor

    Tim Stasevich

    Article

    Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

    Insert

    smHA_KDM5B_24xMS2
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSWAP_Entry_HB

    Plasmid

    #184868

    Purpose

    pSwap plasmid part with HB homology arms

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. TFORF1917

    Plasmid

    #143554

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUNX2 (RUNX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GPRC6A-DuET

    Plasmid

    #213299

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPRC6A (GPRC6A Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-DIO-PPO-Venus (AAV9)

    Viral Prep

    #139505-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Promoter

    EF1a

    Tags

    Venus (Cre-dependent)

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pPEgRNA

    Plasmid

    #172716

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    gRNA
    

    Expression

    Bacterial

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-hISG15

    Plasmid

    #12447

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15 fragment (ISG15 Human)
    

    Expression

    Mammalian

    Mutation

    ISG15 up to LRLRGG

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMaCTag-P11

    Plasmid

    #120022

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet-i
    

    Use

    Pcr template

    Tags

    mScarlet-i

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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