Skip to main content
Addgene

We narrowed to 9,408 results for: Uty

Showing: 801 - 820 of 9408 results
  1. pSEVA251_ Ptrc.x.tetO2::D-HicDH

    Plasmid
    #185456
    Purpose
    CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.
    Depositor
    Insert
    D-HicDH from Lactobacillus paracasei DSM 20008
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptrc.x.tetO2
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pCB’ CsoS2 ΔNTD

    Plasmid
    #162705
    Purpose
    Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2
    Depositor
    Insert
    pHnCB10 derived carboxysome operon, prk
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Deletion of residues 2-256 of CsoS2, Y131D
    Promoter
    PLtet0-1 promoter
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pCCM’ CbbQ-

    Plasmid
    #162711
    Purpose
    Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase
    Depositor
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Inactivation of CbbQ subunit of CbbOQ rubisco act…
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pCCM’ ΔacRAF

    Plasmid
    #162710
    Purpose
    Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF
    Depositor
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    pCCM' with a deletion of putative rubisco ch…
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pSHDY*in_PpetE:BCD2:MrBBS:Strep

    Plasmid
    #133973
    Purpose
    Heterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.
    Depositor
    Insert
    MrBBS:StrepII
    Use
    Synthetic Biology
    Tags
    StrepII
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pCEP4-HA-Flag-natT1R2 RR837-8GG

    Plasmid
    #113958
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    residues 22-839, R837G and R838G substitution
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pMIA10.7

    Plasmid
    #214451
    Purpose
    BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.
    Depositor
    Inserts
    Tet-On 3G
    PuroR
    TRE3G CopGFP
    Use
    Bxbi donor
    Tags
    PuroR, T2A, and Tet-On 3G
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pUCmini-iCAP-AAV9.452sub.LUNG1

    Plasmid
    #184592
    Purpose
    non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection
    Depositor
    Insert
    Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    July 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. attB53-Pac-TRE-3xFlag-Neurog1-attB53

    Plasmid
    #183613
    Purpose
    RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).
    Depositor
    Insert
    Neurog1 (Neurog1 Mouse)
    Use
    Rmce
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 801 - 820 of 9408 results