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Plasmid#154232PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbABDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM2_35_p40S_QfDBD_Qf2AD_cp_nirA_DsRed_5xQUAS
Plasmid#154244PurposeEncodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_nirADepositorInsertp40S:QfDBD-Qf2AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM2_40_pgndA_QfDBD_VP16AD_cp_ura3_DsRed_5xQUAS
Plasmid#154240PurposeEncodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_ura3DepositorInsertpgndA:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterpGndA (An11g02040 A. niger)Available sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_7_p40S_QfDBD_VP16AD_cp_phl_DsRed_5xQUAS
Plasmid#154234PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_phlDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM2_45_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS
Plasmid#154225PurposeEncodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoterDepositorInsertpgndA:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterpGndA (An11g02040 A. niger)Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:GLuc:bgH
Plasmid#135294PurposeTranscriptional unit for constitutive expression of GLuc in mammalian cellsDepositorInsertCMV:GLuc:bGH
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:RoLuc:bgH
Plasmid#135290PurposeTranscriptional unit for constitutive expression of RoLuc in mammalian cellsDepositorInsertCMV:RoLuc:bGH
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:MetLuc:bgH
Plasmid#135292PurposeTranscriptional unit for constitutive expression of MetLuc in mammalian cellsDepositorInsertCMV:MetLuc:bGH
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLM-AMA18.0 dCas9-VPR
Plasmid#138945PurposeFungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markersDepositorInsertp40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
UseCRISPR and Synthetic Biology; Ama1 autonomously r…TagsNLSExpressionMutationPromoterp40S (AN0465) Aspergillus nidulansAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-β′-COPI-WD40-Lys17Ala
Plasmid#208171PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)DepositorInsertN-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution
UseTagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialMutationLys17Ala substitutionPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala
Plasmid#208172PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationArg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala
Plasmid#208173PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
UseTagsStrep-tagExpressionMammalianMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala
Plasmid#208174PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationLys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VcnTS-E1015A-E1021A
Plasmid#213411PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.DepositorInsertVcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
UseLentiviralTagsExpressionMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.165
Plasmid#119634PurposeLevel 0 Part. Insertion siteDepositorInsert6803 NS1 Up Flank
UseSynthetic BiologyTagsExpressionMutation1 nucleotide insertion between nt 21 and 22 in NS…PromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-VIOL
Plasmid#53087PurposeProduces the carotenoid violaxanthin in E. coli.DepositorInsertzep (ABA1 Mustard Weed)
UseLow copy number bacterial cloning vectorTagsExpressionMutationLacks codons for the first 60 N-terminal amino ac…PromoterT7Available sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRa04_1617
Plasmid#49658DepositorInsertECF04_1617
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-17-T
Plasmid#124677PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-8-G
Plasmid#124675PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-19-G
Plasmid#124676PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only