We narrowed to 13,831 results for: sequence
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Plasmid#177807PurposeThe pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-9/9*/124
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP FAT
Plasmid#50517PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertprotein tyrosine kinase, domain
TagsGFPExpressionMammalianMutationtruncated (NT2630-3268)PromoterCMVAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)
Plasmid#181790PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RIPR-bio-His
Plasmid#50824PurposeExpresses enzymatically monobiotinylated full length RIPRDepositorInsertRIPR
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
mito-yHS1
Plasmid#159153PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1
TagsCOX4 pre-sequenceExpressionYeastPromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
gRNAU6
Plasmid#135963PurposeContains tracrRNA-hU6 sequence for double sgRNA cloningDepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)
Plasmid#191162PurposeExpression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry outputDepositorInsertmTagBFP:s70587:mCherry
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-ECFP
Plasmid#48353PurposeContributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertECFP
UseGateway entry vectorMutationContains a stop codon.PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(-)2G
Plasmid#62799PurposemtZFN2G architecture bearing (-) FokI domainDepositorInsertmtZFN(-)2G
TagsInternal FLAG tag immediately after F1B mitochond…ExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(+)2G
Plasmid#62798PurposemtZFN2G architecture bearing (+) FokI domainDepositorInsertmtZFN(+)2G
TagsInternal HA tag immediately after F1B mitochondri…ExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_peroxisome_C1
Plasmid#85064PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
TagsmScarlet-HExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ePEG-LINE1-loxPsym
Plasmid#216866PurposeLentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elementsDepositorInsertspacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
UseCRISPR and LentiviralPromoterU6Available SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHeCAHS-mEGFP-NLS
Plasmid#205014PurposeThe vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRC1765
Plasmid#141346PurposeMini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sapphireAKAR
Plasmid#118466Purposecp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.DepositorInsertLyn-sapphireAKAR
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)
Plasmid#181787PurposeExpresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTnsC, ShCas12k-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only