We narrowed to 14,654 results for: NTS;

1. pAG8H-EWS_1-264

   Plasmid

   #180467

   Purpose

   Expresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tag

   Depositor

   David Libich

   Article

   Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted 265-656

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS699

   Plasmid

   #168779

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD (S betacoronavirus)
   

   Tags

   Cmyc

   Mutation

   N343Q

   Promoter

   GAL1

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PV225

   Plasmid

   #132334

   Purpose

   dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
   

   Tags

   AT-CBF1

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE4560

   Plasmid

   #107526

   Purpose

   Expresses As crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   mCherry
   

   Expression

   Mammalian

   Promoter

   CBh and human U6

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRF+275Dux4

   Plasmid

   #21293

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX 4 (DUX4 Human, DUX 4)
   

   Use

   Luciferase

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pRC/CMV-TYK2P1104A-VSV

   Plasmid

   #139350

   Purpose

   Expression of the TYK2-P1104A variant, rs34536443

   Depositor

   Sandra Pellegrini

   Article

   Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-P1104A

   Promoter

   CMV

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-p53 (1-73) (72P)

   Plasmid

   #62083

   Purpose

   Expression of human p53 (1-73) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72P

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pYPQ262m

   Plasmid

   #161522

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   TadA(wt)-TadA(7.10)-zSpCas9(D10A)
   

   Use

   CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE Tight ArchT-GFP

   Plasmid

   #79627

   Purpose

   Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

   Depositor

   Yasunori Hayashi

   Article

   Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

   Insert

   ArchT
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   ArchT is a more sensitive version of Arch (archae…

   Promoter

   Tet responsive Ptight promoter

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pDONR221 NLRP1 A66V

    Plasmid

    #166817

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    A66V

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cry2 mCherry PGK-1 in pcDNA3.1

    Plasmid

    #64214

    Purpose

    mCherry fused between Cry2 and PGK-1 to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    PGK1 (PGK1 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCEP4-myc-HLA-A2-VN

    Plasmid

    #135485

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus-Bik-pEGFP-C1

    Plasmid

    #166737

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Bik (BIK Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-G2ZYP2

    Plasmid

    #103110

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-mqgDUX4

    Plasmid

    #21176

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pUC19-Q927P4

    Plasmid

    #103131

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. (517) SB KRAB-SadCas9 U6-gStop

    Plasmid

    #163023

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Promoter

    Tet-On

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126778

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FB007

    Plasmid

    #119705

    Purpose

    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Promoter

    gpdA

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ZIKV_NASBA_32B

    Plasmid

    #75011

    Purpose

    Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_32B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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