We narrowed to 89,980 results for: Mal

1. pCMV-myc-Sema4A

   Plasmid

   #115171

   Purpose

   Expresses membrane-targeted Sema4A with N-terminal myc tag

   Depositor

   Suzanne Paradis

   Article

   McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

   Insert

   Sema4A (Sema4a Mouse)
   

   Tags

   membrane targeting sequence and myc

   Expression

   Mammalian

   Mutation

   First 32 amino acids of Sema4A replaced with memb…

   Promoter

   CMV

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-IP-EGFP-ULK1(1-828)

   Plasmid

   #38198

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 829-1051

   Available Since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

   Plasmid

   #69227

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. cycLuc_SbMVs

   Plasmid

   #119209

   Purpose

   Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   cyclic luciferase with SbMVp cleavage site
   

   Use

   Luciferase

   Tags

   AU1 tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTEV5_ RpMatB_4

   Plasmid

   #78208

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A R286C T303A Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A R286C T303A Q457C K488A

   Promoter

   T7

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTEV5_ RpMatB_3

   Plasmid

   #78207

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A T167A R286C Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A T167A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAF216

   Plasmid

   #52405

   Purpose

   Expresses Flag-tagged mouse Arf4

   Depositor

   Gregory Pazour

   Article

   Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

   Insert

   Arf4 (Arf4 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-CaMKIIa-ChRger3-TS-EYFP

   Plasmid

   #127241

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   CamKIIa

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-fSFRP2-GFP

   Plasmid

   #22915

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   fugu secreted frizzled receptor protein 2 promoter driving GFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pMIII-Neo-E2Crimson

    Plasmid

    #153425

    Purpose

    Retroviral (MSCV) expression of E2-Crimson fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    E2-Crimson
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK3_001

    Plasmid

    #123952

    Purpose

    Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3XNLS::CRY2PHR::p65
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK233 (AAVS1 CMV-AtAFB2)

    Plasmid

    #140537

    Purpose

    AAVS1 CMV-AtAFB2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-AtAFB2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5FRT/TO-p97-RH-mycStrep

    Plasmid

    #31839

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    R155H

    Promoter

    CMV

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

    Plasmid

    #81206

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLAMP1-miRFP718nano

    Plasmid

    #195749

    Purpose

    LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    LAMP1-miRFP718nano
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pActin-miRFP718nano

    Plasmid

    #195745

    Purpose

    Human beta-actin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human beta-actin
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2353_Tier3(PB)-YPet_PuroR

    Plasmid

    #169652

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCALNL-eGFP-NT1-6-gix-6-NT1

    Plasmid

    #81204

    Purpose

    contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Expression

    Mammalian

    Promoter

    pCBa

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.2/V5 mmu-mir-153

    Plasmid

    #26330

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-153 (Mir153 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

20. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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