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pAAV-hsyn-GRAB_ATP1.0mut

Plasmid

#167578

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC23A2

Plasmid

#132025

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC23A2 (SLC23A2 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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NES-mTagBFP2-P4Mx1

Plasmid

#108142

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pXL-BACII-attPGAL4LWL

Plasmid

#26260

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-Luc

Plasmid

#25739

Purpose

Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5F Smad1

Plasmid

#12622

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad1 (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only
pCEP4-PDGFRA-reporter

Plasmid

#136456

Purpose

Mammalian fluorescent reporter plasmid for PDGFRA signaling.

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Inserts

PDGFRalpha (PDGFRA Human)
Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV and Minimal promoter with artificial SRE enha…

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSIN-hSNCA-NE

Plasmid

#102366

Purpose

Lentiviral overexpression of synuclein alpha

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Insert

synuclein alpha (SNCA Human)

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

A silent mutation (333bp downstream of ATG) was i…

Promoter

EF-1a

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pv6_MCPH1-TandemBRCT

Plasmid

#250354

Purpose

Expresses tandem BRCT domain from MCPH1 fused to eGFP. Can be used to detect yH2AX at DNA damage sites.

Depositor

Tuncay Baubec

Article

da Silva et al Nat Commun. 2025 Nov 20;16(1):10127. doi

Insert

MCPH1 tandem BRCT

Tags

NLS, eGFP and biotintag, TEV cleavage

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax

Plasmid

#222138

Purpose

Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 (cas9 Synthetic)

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Promoter

T3

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS

Plasmid

#185458

Purpose

Inducible FLAG-tagged mouse cGAS

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec61β

Plasmid

#172445

Purpose

Expression of ER protein Sec61beta with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

Plasmid

#171437

Purpose

Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

c-Fos-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158739

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/TX/50/12 H3

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

G186V S219F

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_FLVCR1

Plasmid

#131892

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

FLVCR1 (FLVCR1 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pQE80L SpyTag-ELP-SpyTag

Plasmid

#112634

Purpose

Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

SpyTag-ELP-SpyTag

Tags

His6

Expression

Bacterial

Mutation

central cysteine

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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cSPBN-4GFP

Plasmid

#52487

Purpose

Expresses EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Protoc. 2010 Mar;5(3):595-606. doi:

Insert

EGFP

Use

Rabies virus

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Nck1

Plasmid

#45892

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 (NCK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only